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Literature summary extracted from

  • Zhang, S.; Kongaeree, P.; Clardy, J.; Wilson, D.B.; Ganem, B.
    Site-directed mutagenesis of monofunctional chorismate mutase engineered from the E. coli P-protein (1996), Bioorg. Med. Chem., 4, 1015-1020.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.5 K39N the mutant enzymes Lys39Arg, Lys39Asn, Lys39Gln, Gln88Arg, and Gln88Glu show similar structures to the wild-type enzyme, as indicated by circular dichroism spectra, with Lys39Gln showing small deviation. The turnover numbers for the mutant enzymes Lys39Arg, Lys39Asn and Lys39Gln are 335fold, 820fold and 4090fold lower than the turnover number of the wild-type enzyme, no significant differences in Km-value for chorismate between Lys39Arg, Lys39Asn, and the wild-type enzyme Escherichia coli
5.4.99.5 K39Q the mutant enzymes Lys39Arg, Lys39Asn, Lys39Gln, Gln88Arg, and Gln88Glu show similar structures to the wild-type enzyme, as indicated by circular dichroism spectra, with Lys39Gln showing small deviation. The turnover numbers for the mutant enzymes Lys39Arg, Lys39Asn and Lys39Gln are 335fold, 820fold and 4090fold lower than the turnover number of the wild-type enzyme, no significant differences in Km-value for chorismate between Lys39Arg, Lys39Asn, and the wild-type enzyme Escherichia coli
5.4.99.5 K39R the mutant enzymes Lys39Arg, Lys39Asn, Lys39Gln, Gln88Arg, and Gln88Glu show similar structures to the wild-type enzyme, as indicated by circular dichroism spectra, with Lys39Gln showing small deviation. The turnover numbers for the mutant enzymes Lys39Arg, Lys39Asn and Lys39Gln are 335fold, 820fold and 4090fold lower than the turnover number of the wild-type enzyme, no significant differences in Km-value for chorismate between Lys39Arg, Lys39Asn, and the wild-type enzyme Escherichia coli
5.4.99.5 Q88E the mutant enzymes Lys39Arg, Lys39Asn, Lys39Gln, Gln88Arg, and Gln88Glu show similar structures to the wild-type enzyme, as indicated by circular dichroism spectra, with Lys39Gln showing small deviation. The turnover numbers for the mutant enzymes Lys39Arg, Lys39Asn and Lys39Gln are 335fold, 820fold and 4090fold lower than the turnover number of the wild-type enzyme, no significant differences in Km-value for chorismate between Lys39Arg, Lys39Asn, and the wild-type enzyme Escherichia coli
5.4.99.5 Q88E mutation of Gln88 to Glu in the monofunctional chorismate mutase results in an enzyme with a pH profile of activity significantly different from that of the wild-type protein Escherichia coli
5.4.99.5 Q88R the mutant enzymes Lys39Arg, Lys39Asn, Lys39Gln, Gln88Arg, and Gln88Glu show similar structures to the wild-type enzyme, as indicated by circular dichroism spectra, with Lys39Gln showing small deviation. The turnover numbers for the mutant enzymes Lys39Arg, Lys39Asn and Lys39Gln are 335fold, 820fold and 4090fold lower than the turnover number of the wild-type enzyme, no significant differences in Km-value for chorismate between Lys39Arg, Lys39Asn, and the wild-type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.3
-
 chorismate wild-type enzyme Escherichia coli
5.4.99.5 0.45
-
 chorismate mutant enzyme Lys39Asn Escherichia coli
5.4.99.5 0.59
-
 chorismate mutant enzyme Lys39Arg Escherichia coli
5.4.99.5 1.16
-
 chorismate mutant enzyme Lys39Gln Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Escherichia coli
-
 mutant enzymes Lys39Arg, Lys39Asn, Lys39Gln, Gln88Arg, and Gln88Glu
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Escherichia coli Prephenate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 0.00983
-
 chorismate mutant enzyme Lys39Arg Escherichia coli
5.4.99.5 0.0492
-
 chorismate mutant enzyme Lys39Asn Escherichia coli
5.4.99.5 0.123
-
 chorismate mutant enzyme Lys39Arg Escherichia coli
5.4.99.5 41.4
-
 chorismate wild-type enzyme Escherichia coli