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Literature summary extracted from
- Chorismate mutase isoenzymes from Sorghum bicolor: purification and properties (1985), Arch. Biochem. Biophys., 243, 374-384.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.5 | L-Trp | activates | Sorghum bicolor |  |
| 5.4.99.5 | L-Trp | enzyme form CM2 is unaffected | Sorghum bicolor | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.5 | L-Phe | enzyme form CM1 is inhibited; enzyme form CM2 is not inhibited | Sorghum bicolor | |
| 5.4.99.5 | L-Tyr | enzyme form CM2 is not inhibited | Sorghum bicolor | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 48000 | - |
enzyme form CM2, gel filtration | Sorghum bicolor |
| 5.4.99.5 | 56000 | - |
enzyme form CM1, gel filtration | Sorghum bicolor |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | Sorghum bicolor | - |
2 enzyme forms: CM1 and CM2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | enzyme form CM1 and CM2 | Sorghum bicolor |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | culm | - |
Sorghum bicolor | - |
| 5.4.99.5 | kernel | - |
Sorghum bicolor | - |
| 5.4.99.5 | leaf | - |
Sorghum bicolor | - |
| 5.4.99.5 | seedling | green or etiolated | Sorghum bicolor | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.99.5 | 0°C or -20°C, enzyme forms CM1 and CM2, stable for at least 6 weeks | Sorghum bicolor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | - |
Sorghum bicolor | Prephenate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 55 | - |
pH 9.0, 5 min, enzyme form CM2, 15% loss of activity | Sorghum bicolor |
| 5.4.99.5 | 60 | - |
pH 9.0, 5 min, stable up to | Sorghum bicolor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 6 | 10 | enzyme form CM1 | Sorghum bicolor |
| 5.4.99.5 | 7.5 | 9.5 | enzyme form CM2 | Sorghum bicolor |
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