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Literature summary extracted from
- Separation and characterization of two chorismate-mutase isoenzymes from Nicotiana silvestris (1984), Planta, 162, 109-116.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 0.24 | - |
chorismate | enzyme form CM2 | Nicotiana sylvestris |  |
| 5.4.99.5 | 1.7 | - |
chorismate | enzyme form CM1, weak positive cooperativity | Nicotiana sylvestris | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.4.99.5 | cytosol | enzyme form CM2 | Nicotiana sylvestris | 5829 | - |
| 5.4.99.5 | plastid | chloroplasts or proplastids, enzyme form CM1 | Nicotiana sylvestris | 9536 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 52000 | - |
enzyme form CM1, gel filtration | Nicotiana sylvestris |
| 5.4.99.5 | 65000 | - |
enzyme form CM2, gel filtration | Nicotiana sylvestris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | Nicotiana sylvestris | - |
2 enzyme forms: CM1 and CM2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | partial | Nicotiana sylvestris |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | cell suspension culture | the major fraction is formed by enzyme form CM2 | Nicotiana sylvestris | - |
| 5.4.99.5 | leaf | major fraction is formed by enzyme form CM1 | Nicotiana sylvestris | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.99.5 | 0°C or -20°C, enzyme form CM1, stable for at least 1 month | Nicotiana sylvestris |
| 5.4.99.5 | 0°C or -20°C, enzyme form CM2, stable for at least 2 weeks | Nicotiana sylvestris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | - |
Nicotiana sylvestris | Prephenate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 45 | - |
20 min, no denaturation, enzyme form CM1 and CM2 | Nicotiana sylvestris |
| 5.4.99.5 | 52 | - |
20 min, 50% loss of activity, enzyme form CM1 | Nicotiana sylvestris |
| 5.4.99.5 | 52 | - |
5 min, more than 50% loss of activity, enzyme form CM2 | Nicotiana sylvestris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 6 | 8 | enzyme form CM1 and CM2 | Nicotiana sylvestris |
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