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Literature summary extracted from

  • Friedrich, B.; Friedrich, C.G.; Schlegel, H.G.
    Purification and properties of chorismate mutase-prephenate dehydratase and prephenate dehydrogenase from Alcaligenes eutrophus (1976), J. Bacteriol., 126, 712-722.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.3.1.12 glycerol stabilizes Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.12 Cu2+
-
 Cupriavidus necator
1.3.1.12 Fe2+
-
 Cupriavidus necator
1.3.1.12 Hg2+
-
 Cupriavidus necator
1.3.1.12 Mg2+
-
 Cupriavidus necator
4.2.1.51 Cu2+ 0.1 mM, 100% inhibition Cupriavidus necator
4.2.1.51 Fe2+ 0.1 mM, 85% inhibition Cupriavidus necator
4.2.1.51 Hg2+ 0.1 mM, 94% inhibition Cupriavidus necator
4.2.1.51 Ni2+ 0.1 mM, 83% inhibition Cupriavidus necator
4.2.1.51 Zn2+ 0.1 mM, 58% inhibition Cupriavidus necator
5.4.99.5 Cu2+
-
 Cupriavidus necator
5.4.99.5 Fe2+
-
 Cupriavidus necator
5.4.99.5 Hg2+
-
 Cupriavidus necator
5.4.99.5 Ni2+
-
 Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.12 55000 65000 gel filtration, gel electrophoresis, sucrose density gradient centrifugation Cupriavidus necator
4.2.1.51 144000
-
 ultracentrifugation Cupriavidus necator
4.2.1.51 187000
-
 gel filtration Cupriavidus necator
5.4.99.5 47000
-
 4 * 47000, chorismate mutase/prephenate dehydratase, SDS-PAGE Cupriavidus necator
5.4.99.5 160000 180000 disc gel electrophoresis Cupriavidus necator
5.4.99.5 187000
-
 chorismate mutase/prephenate dehydratase, gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.12 prephenate + NAD+ Cupriavidus necator biosynthesis of L-tyrosine 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?
4.2.1.51 prephenate Cupriavidus necator
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.12 Cupriavidus necator
-
-
-
4.2.1.51 Cupriavidus necator
-
-
-
5.4.99.5 Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.12 not associated with other enzymatic activities Cupriavidus necator
4.2.1.51 bifunctional enzyme, chorismate mutase:prephenate dehydratase Cupriavidus necator
5.4.99.5 in association with prephenate dehydratase Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.12 additional information
-
-
 Cupriavidus necator
1.3.1.12 8.9
-
-
 Cupriavidus necator
4.2.1.51 24.9
-
-
 Cupriavidus necator
5.4.99.5 36.63
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
1.3.1.12 -20°C, 50 mM phosphate buffer, pH 7.4, at least 0.7 mg/ml protein, 10 months Cupriavidus necator
4.2.1.51 -20°C, 50 mM phosphate buffer, pH 7.4, 0.7 mg protein/ml, stable for 10 months Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.12 prephenate + NAD+
-
 Cupriavidus necator 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?
1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Cupriavidus necator 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?
4.2.1.51 prephenate
-
 Cupriavidus necator phenylpyruvate + H2O + CO2
-
 ?
4.2.1.51 prephenate
-
 Cupriavidus necator ?
-
 ?
5.4.99.5 Chorismate
-
 Cupriavidus necator Prephenate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 tetramer 4 * 47000, chorismate mutase/prephenate dehydratase, SDS-PAGE Cupriavidus necator