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Literature summary extracted from

  • Haagen, L.; Brock, A.
    A new automated method for phenotyping arylesterase (EC 3.1.1.2) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate (1992), Eur. J. Clin. Chem. Clin. Biochem., 30, 391-395.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.2 analysis description of an easy and non-toxic method for large scale phenotyping and activity quantitation of arylesterase Homo sapiens
3.1.8.1 analysis description of an easy and non-toxic method for large scale phenotyping and activity quantitation of arylesterase Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 phenyl acetate inhibits hydrolysis of 4-nitrophenyl acetate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 additional information
-
 additional information Km-values in serum samples Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens
-
-
-
3.1.8.1 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 blood serum
-
 Homo sapiens
-
3.1.8.1 blood serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Homo sapiens 4-nitrophenol + acetate
-
 ?
3.1.1.2 phenyl acetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?
3.1.8.1 paraoxon + H2O
-
 Homo sapiens 4-nitrophenol + diethylphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.2 25
-
 assay at Homo sapiens
3.1.8.1 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.2 8
-
 assay at Homo sapiens
3.1.8.1 10.5
-
 assay at Homo sapiens