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Literature summary extracted from
- Biosynthesis of isoleucine and valine in Mycobacterium tuberculosis H37 Rv (1968), Arch. Biochem. Biophys., 125, 199-209.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.3 | ascorbic acid | required, D-ascorbate shows 80% of L-ascorbate in enhancing the reaction, cannot be replaced by other reducing agents | Mycobacterium tuberculosis |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.3 | 2.4 | - |
2-acetolactate | - |
Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.3 | 2-Acetolactate | Mycobacterium tuberculosis | enzyme of Ile-Val biosynthesis | ? | - |
? | |
| 5.4.99.3 | 2-Acetolactate | Mycobacterium tuberculosis H37Rv | enzyme of Ile-Val biosynthesis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.3 | Mycobacterium tuberculosis | - |
H37Rv | - |
| 5.4.99.3 | Mycobacterium tuberculosis H37Rv | - |
H37Rv | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.99.3 | 90% loss of activity after 2 days, even when kept under frozen state | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.3 | 2-aceto-2-hydroxybutanoate | - |
Mycobacterium tuberculosis | 3-hydroxy-3-methyl-2-oxopentanoate | - |
? | |
| 5.4.99.3 | 2-aceto-2-hydroxybutanoate | - |
Mycobacterium tuberculosis H37Rv | 3-hydroxy-3-methyl-2-oxopentanoate | - |
? | |
| 5.4.99.3 | 2-Acetolactate | - |
Mycobacterium tuberculosis | 3-Hydroxy-3-methyl-2-oxobutanoate | - |
? | |
| 5.4.99.3 | 2-Acetolactate | - |
Mycobacterium tuberculosis H37Rv | 3-Hydroxy-3-methyl-2-oxobutanoate | - |
? | |
| 5.4.99.3 | 2-Acetolactate | enzyme of Ile-Val biosynthesis | Mycobacterium tuberculosis | ? | - |
? | |
| 5.4.99.3 | 2-Acetolactate | enzyme of Ile-Val biosynthesis | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.3 | 7.5 | - |
- |
Mycobacterium tuberculosis |
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