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Literature summary extracted from

  • Rauhut, R.; Gabius, H.J.; Cramer, F.
    Phenylalanyl-tRNA synthetase from chloroplast of a higher plant (Phaseolus vulgaris). Purification and comparison of its structural, functional, and immunological properties with those of the enzymes from the corresponding cytoplasm, the cyanobacterium Anacystis nidulans, and the photosynthetic green sulfur bacterium Chlorobium limicola (1986), J. Biol. Chem., 261, 2799-2803.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.20 additional information
-
 additional information
-
 Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.20 chloroplast
-
 Phaseolus vulgaris 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.20 33000
-
 1 * 33000 (alpha) + 1 * 42000 (beta), SDS-PAGE Phaseolus vulgaris
6.1.1.20 42000
-
 1 * 33000 (alpha) + 1 * 42000 (beta), SDS-PAGE Phaseolus vulgaris
6.1.1.20 78000
-
 gel filtration Phaseolus vulgaris
6.1.1.20 260000
-
-
 Phaseolus vulgaris
6.1.1.20 260000
-
 gel filtration Synechococcus elongatus PCC 7942 = FACHB-805
6.1.1.20 260000
-
 gel filtration Chlorobium limicola

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Chlorobium limicola
-
-
-
6.1.1.20 Phaseolus vulgaris
-
-
-
6.1.1.20 Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.20
-
 Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.20 leaf
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.20 0.0107
-
-
 Chlorobium limicola
6.1.1.20 0.092
-
-
 Synechococcus elongatus PCC 7942 = FACHB-805
6.1.1.20 0.11
-
-
 Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Phaseolus vulgaris AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Synechococcus elongatus PCC 7942 = FACHB-805 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Chlorobium limicola AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.20 dimer 1 * 33000 (alpha) + 1 * 42000 (beta), SDS-PAGE Phaseolus vulgaris