BRENDA - Enzyme Database

Inhibition of the human methylmalonyl-CoA mutase by various CoA-esters

Taoka, S.; Padmakumar, R.; Lai, M.; Liu, H.; Banerjee, R.; J. Biol. Chem. 269, 31630-31634 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.2
Cyclopropylcarbonyl-CoA carboxylate
reversible mixed-type inhibition
Homo sapiens
5.4.99.2
methylenecyclopropylacetyl-CoA
reversible mixed-type inhibition
Homo sapiens
5.4.99.2
methylmalonyl-CoA
reversible mixed-type inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.2
0.06
-
(R/S)-2-methyl-3-oxopropanoyl-CoA
-
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
5.4.99.2
Homo sapiens
-
expressed in Saccharomyces cerevisiae
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
5.4.99.2
expressed in Saccharomyces cerevisiae
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.99.2
67.4
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5.4.99.2
(R)-2-methylmalonyl-CoA
-
3513
Homo sapiens
succinyl-CoA
-
3513
Homo sapiens
?
5.4.99.2
(R/S)-2-methyl-3-oxopropanoyl-CoA
-
3513
Homo sapiens
succinyl-CoA
-
-
-
?
5.4.99.2
ethylmalonyl-CoA
reacts about 1000-10000 times moreslowly than the natural substrate
3513
Homo sapiens
methylsuccinyl-CoA
-
3513
Homo sapiens
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.99.2
150
-
(R/S)-2-methyl-3-oxopropanoyl-CoA
30°C
Homo sapiens
5.4.99.2
283
-
(R/S)-2-methyl-3-oxopropanoyl-CoA
37°C
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.2
Cyclopropylcarbonyl-CoA carboxylate
reversible mixed-type inhibition
Homo sapiens
5.4.99.2
methylenecyclopropylacetyl-CoA
reversible mixed-type inhibition
Homo sapiens
5.4.99.2
methylmalonyl-CoA
reversible mixed-type inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.2
0.06
-
(R/S)-2-methyl-3-oxopropanoyl-CoA
-
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.2
expressed in Saccharomyces cerevisiae
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.99.2
67.4
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5.4.99.2
(R)-2-methylmalonyl-CoA
-
3513
Homo sapiens
succinyl-CoA
-
3513
Homo sapiens
?
5.4.99.2
(R/S)-2-methyl-3-oxopropanoyl-CoA
-
3513
Homo sapiens
succinyl-CoA
-
-
-
?
5.4.99.2
ethylmalonyl-CoA
reacts about 1000-10000 times moreslowly than the natural substrate
3513
Homo sapiens
methylsuccinyl-CoA
-
3513
Homo sapiens
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.99.2
150
-
(R/S)-2-methyl-3-oxopropanoyl-CoA
30°C
Homo sapiens
5.4.99.2
283
-
(R/S)-2-methyl-3-oxopropanoyl-CoA
37°C
Homo sapiens