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Literature summary extracted from
- Methylmalonyl-CoA mutase (1982), B12 (Dolphin, D. ed. ) Wiley, New York, 2, 357-379.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | NEM | - |
Mammalia |  |
| 5.4.99.2 | p-hydroxymercuribenzoate | - |
Mammalia | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.2 | 0.0345 | - |
succinyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | 0.062 | - |
succinyl-CoA | - |
Ovis aries | |
| 5.4.99.2 | 0.085 | - |
(R)-2-methyl-3-oxopropanoyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | 0.24 | - |
(R)-2-methyl-3-oxopropanoyl-CoA | - |
Ovis aries | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 61000 | - |
1 * 61000 + 1 * 66000, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |
| 5.4.99.2 | 66000 | - |
1 * 61000 + 1 * 66000, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |
| 5.4.99.2 | 122000 | 124000 | gel filtration, calculation from sedimentation and diffusion data | Propionibacterium freudenreichii subsp. shermanii |
| 5.4.99.2 | 165000 | - |
determination from sedimentation data | Ovis aries |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | (R)-2-Methyl-3-oxopropanoyl-CoA | Ovis aries | key position in metabolism of propionic acid | ? | - |
? | |
| 5.4.99.2 | Succinyl-CoA | Propionibacterium freudenreichii subsp. shermanii | production of propionate from succinate | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.2 | Mammalia | - |
- |
- |
| 5.4.99.2 | Ovis aries | - |
- |
- |
| 5.4.99.2 | Propionibacterium freudenreichii subsp. shermanii | - |
strain 52W and strain St 33 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.2 | - |
Ovis aries |
| 5.4.99.2 | - |
Propionibacterium freudenreichii subsp. shermanii |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.4.99.2 | (R)-methylmalonyl-CoA = succinyl-CoA | mechanism | Ovis aries | |
| 5.4.99.2 | (R)-methylmalonyl-CoA = succinyl-CoA | mechanism | Propionibacterium freudenreichii subsp. shermanii |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.2 | liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | additional information | - |
- |
Ovis aries |
| 5.4.99.2 | additional information | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.99.2 | -10°C, enzyme from strain 52 W is quite stable, the enzyme from strain St 33 is much less so | Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | (R)-2-Methyl-3-oxopropanoyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
| 5.4.99.2 | (R)-2-Methyl-3-oxopropanoyl-CoA | key position in metabolism of propionic acid | Ovis aries | ? | - |
? | |
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Mammalia | succinyl-CoA | - |
? | |
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Ovis aries | succinyl-CoA | - |
? | |
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | succinyl-CoA | - |
? | |
| 5.4.99.2 | Succinyl-CoA | production of propionate from succinate | Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.2 | dimer | 1 * 61000 + 1 * 66000, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 7 | - |
- |
Ovis aries |
| 5.4.99.2 | 7.4 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | Coalpha(alpha-Benzimidazolyl)-Cobeta-adenosyl-cobamide | can serve as coenzyme | Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | Coalpha-(alpha-Purinyl)-Cobeta-adenosylcobamide | can serve as coenzyme | Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | Coalpha-Hydroxo-Cobeta-adenosylcobinamide | can serve as coenzyme | Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | Coalpha[alpha-(Aden-7-yl)]-Cobeta-adenosyl-cobamide | can serve as coenzyme | Propionibacterium freudenreichii subsp. shermanii | |
| 5.4.99.2 | cobamide | - |
Mammalia | |
| 5.4.99.2 | cobamide | adenosylcobalamin protects the apoenzyme from inactivation by NEM or iodoacetamide. Km: 0.000021 mM | Ovis aries | |
| 5.4.99.2 | cobamide | one molecule of enzyme contains about 2 molecules of adenosylcobalamin | Ovis aries | |
| 5.4.99.2 | cobamide | Km: 0.000035 mM | Propionibacterium freudenreichii subsp. shermanii | |
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Release 2023.1 (January 2023)
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