EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.6 | additional information | - |
Xanthobacter autotrophicus Py2 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.6 | 0.0078 | - |
acetone | - |
Xanthobacter autotrophicus Py2 | |
6.4.1.6 | 0.122 | - |
ATP | - |
Xanthobacter autotrophicus Py2 | |
6.4.1.6 | 4.17 | - |
CO2 | cosubstrate bicarbonate | Xanthobacter autotrophicus Py2 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.6 | Mg2+ | - |
Xanthobacter autotrophicus Py2 | |
6.4.1.6 | additional information | addition of Fe2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+ or Ni2+ do not stimulate the enzyme activity above the maximal levels obtained in the presence of Mg2+ alone | Xanthobacter autotrophicus Py2 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.6 | 19600 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
6.4.1.6 | 23000 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
6.4.1.6 | 68400 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
6.4.1.6 | 78300 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
6.4.1.6 | 78800 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
6.4.1.6 | 85300 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
6.4.1.6 | 353000 | - |
native molecular weight of enzyme complex | Xanthobacter autotrophicus Py2 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.6 | Xanthobacter autotrophicus Py2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.6 | DEAE-Sepharose chromatography, gel filtration with Sephacryl S-300 column, HiLoad Q-Sepharose column | Xanthobacter autotrophicus Py2 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.4.1.6 | 0.094 | - |
usage of butanone | Xanthobacter autotrophicus Py2 |
6.4.1.6 | 0.225 | - |
usage of acetone at 30°C and pH 7.6 | Xanthobacter autotrophicus Py2 |
EC Number | Storage Stability | Organism |
---|---|---|
6.4.1.6 | 4°C, pH 6.5-8.0, activity stable for several days | Xanthobacter autotrophicus Py2 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.6 | acetone + CO2 + ATP + H2O | - |
Xanthobacter autotrophicus Py2 | acetoacetate + AMP + phosphate | - |
? | |
6.4.1.6 | butanone + CO2 + ATP + H2O | - |
Xanthobacter autotrophicus Py2 | 3-oxopentanoate + AMP + phosphate | - |
? | |
6.4.1.6 | additional information | enzyme expressed by acetone and isopropanol grown cells, not by propylene or glucose grown cells | Xanthobacter autotrophicus Py2 | ? | - |
? | |
6.4.1.6 | additional information | 2-pentanone, 3-pentanone, 2-hexanone and chloroacetone are substrates | Xanthobacter autotrophicus Py2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.6 | trimer | 1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.6 | 30 | - |
- |
Xanthobacter autotrophicus Py2 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.6 | 7.6 | - |
- |
Xanthobacter autotrophicus Py2 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.6 | ATP | obligate requirement | Xanthobacter autotrophicus Py2 |