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Literature summary extracted from

  • Sluis, M.K.; Ensign, S.A.
    Purification and characterization of acetone carboxylase from Xanthobacter strain Py2 (1997), Proc. Natl. Acad. Sci. USA, 94, 8456-8461.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.6 additional information
-
Xanthobacter autotrophicus Py2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.6 0.0078
-
acetone
-
Xanthobacter autotrophicus Py2
6.4.1.6 0.122
-
ATP
-
Xanthobacter autotrophicus Py2
6.4.1.6 4.17
-
CO2 cosubstrate bicarbonate Xanthobacter autotrophicus Py2

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.6 Mg2+
-
Xanthobacter autotrophicus Py2
6.4.1.6 additional information addition of Fe2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+ or Ni2+ do not stimulate the enzyme activity above the maximal levels obtained in the presence of Mg2+ alone Xanthobacter autotrophicus Py2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.6 19600
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 23000
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 68400
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 78300
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 78800
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 85300
-
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2
6.4.1.6 353000
-
native molecular weight of enzyme complex Xanthobacter autotrophicus Py2

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.6 Xanthobacter autotrophicus Py2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.6 DEAE-Sepharose chromatography, gel filtration with Sephacryl S-300 column, HiLoad Q-Sepharose column Xanthobacter autotrophicus Py2

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.6 0.094
-
usage of butanone Xanthobacter autotrophicus Py2
6.4.1.6 0.225
-
usage of acetone at 30°C and pH 7.6 Xanthobacter autotrophicus Py2

Storage Stability

EC Number Storage Stability Organism
6.4.1.6 4°C, pH 6.5-8.0, activity stable for several days Xanthobacter autotrophicus Py2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.6 acetone + CO2 + ATP + H2O
-
Xanthobacter autotrophicus Py2 acetoacetate + AMP + phosphate
-
?
6.4.1.6 butanone + CO2 + ATP + H2O
-
Xanthobacter autotrophicus Py2 3-oxopentanoate + AMP + phosphate
-
?
6.4.1.6 additional information enzyme expressed by acetone and isopropanol grown cells, not by propylene or glucose grown cells Xanthobacter autotrophicus Py2 ?
-
?
6.4.1.6 additional information 2-pentanone, 3-pentanone, 2-hexanone and chloroacetone are substrates Xanthobacter autotrophicus Py2 ?
-
?

Subunits

EC Number Subunits Comment Organism
6.4.1.6 trimer 1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure Xanthobacter autotrophicus Py2

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.6 30
-
-
Xanthobacter autotrophicus Py2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.6 7.6
-
-
Xanthobacter autotrophicus Py2

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.6 ATP obligate requirement Xanthobacter autotrophicus Py2