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Literature summary extracted from
- Purification and characterization of the tetrachloroethene reductive dehalogenase of strain PCE-S (1998), Arch. Microbiol., 169, 497-502.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.21.99.5 | agriculture | suitable for tetrachloroethene bioremediation | Desulfitobacterium hafniense |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.21.99.5 | oxygen-sensitive with half-life of approximately 50 min | Desulfitobacterium hafniense |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | additional information | no inhibition by ammonium ions | Desulfitobacterium hafniense | |
| 1.21.99.5 | Propyl iodide | 1-iodopropane; 1-iodopropane, photoreversible inactivation | Desulfitobacterium hafniense |  |
| 1.21.99.5 | trichloroethene | substrate inhibition, higher concentrations of trichloroethene: inhibition of PCE dehalogenase, Ki: 1.3 mM | Desulfitobacterium hafniense | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.99.5 | additional information | - |
additional information | enzyme kinetics and kinetic parameters | Desulfitobacterium hafniense | |
| 1.21.99.5 | 0.004 | - |
trichloroethene | - |
Desulfitobacterium hafniense | |
| 1.21.99.5 | 0.01 | - |
tetrachloroethene | - |
Desulfitobacterium hafniense | |
| 1.21.99.5 | 0.3 | - |
methyl viologen | at a tetrachloroethene concentration of 1 mM | Desulfitobacterium hafniense | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.21.99.5 | cytoplasmic membrane | membrane-bound | Desulfitobacterium hafniense | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | Cobalamin | dicyano-cob(III)alamin | Desulfitobacterium hafniense | |
| 1.21.99.5 | Cobalt | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
| 1.21.99.5 | Cobalt | cobalt content: 1.0 mol of cobalt/mol of protein | Desulfitobacterium hafniense | |
| 1.21.99.5 | Fe2+ | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
| 1.21.99.5 | Fe2+ | two Fe/S clusters | Desulfitobacterium hafniense | |
| 1.21.99.5 | Fe2+ | two Fe4-S4 clusters | Desulfitobacterium hafniense | |
| 1.21.99.5 | Iron-sulfur cluster | iron-sulfur protein | Desulfitobacterium hafniense | |
| 1.21.99.5 | Iron-sulfur cluster | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
| 1.21.99.5 | Iron-sulfur cluster | two Fe4-S4 clusters | Desulfitobacterium hafniense | |
| 1.21.99.5 | iron-sulfur clusters | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
| 1.21.99.5 | iron-sulfur clusters | two Fe4-S4 clusters | Desulfitobacterium hafniense | |
| 1.21.99.5 | NH4+ | no effect, no activation by ammonium ions | Desulfitobacterium hafniense | |
| 1.21.99.5 | S | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
| 1.21.99.5 | S | two Fe/S clusters | Desulfitobacterium hafniense | |
| 1.21.99.5 | S | two Fe4-S4 clusters | Desulfitobacterium hafniense | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 65000 | - |
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE | Desulfitobacterium hafniense |
| 1.21.99.5 | 200000 | - |
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, gel filtration | Desulfitobacterium hafniense |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | tetrachloroethene + reduced acceptor | Desulfitobacterium hafniense | - |
trichloroethene + chloride + acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.21.99.5 | Desulfitobacterium hafniense | - |
strictly anaerobic, gram-positive bacterium | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.21.99.5 | - |
Desulfitobacterium hafniense |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 39 | - |
- |
Desulfitobacterium hafniense |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | additional information | substrate specificity | Desulfitobacterium hafniense | ? | - |
? | |
| 1.21.99.5 | additional information | enzyme quite specific | Desulfitobacterium hafniense | ? | - |
? | |
| 1.21.99.5 | additional information | very high substrate specificity | Desulfitobacterium hafniense | ? | - |
? | |
| 1.21.99.5 | additional information | no activity with other chloroethenes or chloromethanes or chlorinated aromatic compounds, no activity with carbon tetrachloride, 1,1-dichloroethene, trans-1,2-dichloroethene, cis-1,2-dichloroethene, 3-chlorobenzoate, 3-chloro-4-hydroxyphenol, pentachlorophenol, 2,4,6-trichlorophenol, 2,4,5-trichlorophenol | Desulfitobacterium hafniense | ? | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | - |
Desulfitobacterium hafniense | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | artificial electron donor: reduced methyl viologen, physiological electron donor is not known | Desulfitobacterium hafniense | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Desulfitobacterium hafniense | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | trichloroethene + chloride + methyl viologen | - |
Desulfitobacterium hafniense | tetrachloroethene + reduced methyl viologen | - |
r | |
| 1.21.99.5 | trichloroethene + reduced acceptor | artificial electron donor: reduced methyl viologen, physiological electron donor is not known | Desulfitobacterium hafniense | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | trichloroethene + reduced acceptor | 70% of activity compared with tetrachloroethene | Desulfitobacterium hafniense | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.21.99.5 | monomer | 1 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, probably a monomeric 65-kDa protein, SDS-PAGE | Desulfitobacterium hafniense |
| 1.21.99.5 | More | membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo | Desulfitobacterium hafniense |
| 1.21.99.5 | trimer | 3 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE | Desulfitobacterium hafniense |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 50 | - |
approximately | Desulfitobacterium hafniense |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 7.2 | - |
approximately | Desulfitobacterium hafniense |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | corrinoid | corrinoid protein | Desulfitobacterium hafniense | |
| 1.21.99.5 | corrinoid | dicyano-cobalamin | Desulfitobacterium hafniense | |
| 1.21.99.5 | corrinoid | dicyano-cob(III)alamin | Desulfitobacterium hafniense | |
| 1.21.99.5 | corrinoid | contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit | Desulfitobacterium hafniense | |
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