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Literature summary extracted from

  • Schumacher, W.; Holliger, C.
    The proton/electron ratio of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus" (1996), J. Bacteriol., 178, 2328-2333.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.21.99.5 agriculture degradation of lipophilic solvent tetrachloroethene, one of the most abundant halogenated xenobiotic pollutants in the environment Dehalobacter restrictus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.99.5 Propyl iodide 1-iodopropane; 1-iodopropane, photoreversible inactivation Dehalobacter restrictus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.99.5 additional information
-
 additional information
-
 Dehalobacter restrictus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.21.99.5 cytoplasmic membrane cytoplasmic membrane Dehalobacter restrictus
-
-
1.21.99.5 cytoplasmic membrane cytoplasmic side of membrane, mainly associated with the membrane Dehalobacter restrictus
-
-
1.21.99.5 additional information cellular localization Dehalobacter restrictus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.99.5 Cobalt
-
 Dehalobacter restrictus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.99.5 tetrachloroethene + reduced acceptor Dehalobacter restrictus
-
 trichloroethene + chloride + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.5 Dehalobacter restrictus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.21.99.5 trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor mechanism Dehalobacter restrictus
a
1.21.99.5 trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor thermodynamics Dehalobacter restrictus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.99.5 0.33
-
-
 Dehalobacter restrictus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.5 tetrachloroethene + reduced acceptor
-
 Dehalobacter restrictus trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor lipophilic solvent tetrachloroethene, one of the most abundant halogenated xenobiotic pollutants in the environment Dehalobacter restrictus trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Dehalobacter restrictus trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 trichloroethene + reduced acceptor
-
 Dehalobacter restrictus cis-1,2-dichloroethene + chloride + acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.99.5 30
-
 assay at Dehalobacter restrictus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.99.5 8.1
-
 assay at Dehalobacter restrictus

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.99.5 corrinoid
-
 Dehalobacter restrictus
1.21.99.5 menaquinone membrane-bound menaquinone Dehalobacter restrictus