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Literature summary extracted from

  • Schumacher, W.; Holliger, C.; Zehnder, A.J.B.; Hagen, W.R.
    Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus (1997), FEBS Lett., 409, 421-425.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.99.5 Propyl iodide 1-iodopropane; 1-iodopropane, photoreversible inactivation Dehalobacter restrictus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.21.99.5 cytoplasmic membrane membrane-bound Dehalobacter restrictus
-
-
1.21.99.5 cytoplasmic membrane cytoplasmic membrane Dehalobacter restrictus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.99.5 Cobalamin cob(II)alamin Dehalobacter restrictus
1.21.99.5 Cobalamin in base-off configuration Dehalobacter restrictus
1.21.99.5 Cobalt probably present in the 2+ oxidation state Dehalobacter restrictus
1.21.99.5 Fe2+ two Fe4-S4 clusters Dehalobacter restrictus
1.21.99.5 Iron-sulfur cluster two iron-sulfur cubane cofactors Dehalobacter restrictus
1.21.99.5 Iron-sulfur cluster two Fe4-S4 clusters Dehalobacter restrictus
1.21.99.5 iron-sulfur clusters two Fe4-S4 clusters Dehalobacter restrictus
1.21.99.5 S two Fe4-S4 clusters Dehalobacter restrictus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.99.5 60000
-
SDS-PAGE Dehalobacter restrictus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.99.5 additional information Dehalobacter restrictus PCE reductase is the final enzyme of the perchloroethene respiratory chain that catalyzes the reductive cleavage of a carbon chlorine bond ?
-
?
1.21.99.5 tetrachloroethene + reduced acceptor Dehalobacter restrictus PCE reductase is the final enzyme of the perchloroethene respiratory chain that catalyzes the reductive cleavage of a carbon chlorine bond trichloroethene + chloride + acceptor
-
?
1.21.99.5 trichloroethene + reduced acceptor Dehalobacter restrictus
-
cis-1,2-dichloroethene + chloride + acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.5 Dehalobacter restrictus
-
DSMZ 9455T
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.99.5
-
Dehalobacter restrictus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.21.99.5 trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor mechanism Dehalobacter restrictus
1.21.99.5 trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor chemiosmotic mechanism Dehalobacter restrictus
1.21.99.5 trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor thermodynamics Dehalobacter restrictus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.99.5 11.9
-
-
Dehalobacter restrictus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.5 additional information physiological electron donor is not known Dehalobacter restrictus ?
-
?
1.21.99.5 additional information PCE reductase is the final enzyme of the perchloroethene respiratory chain that catalyzes the reductive cleavage of a carbon chlorine bond Dehalobacter restrictus ?
-
?
1.21.99.5 tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Dehalobacter restrictus trichloroethene + chloride + acceptor
-
?
1.21.99.5 tetrachloroethene + reduced acceptor PCE reductase is the final enzyme of the perchloroethene respiratory chain that catalyzes the reductive cleavage of a carbon chlorine bond Dehalobacter restrictus trichloroethene + chloride + acceptor
-
?
1.21.99.5 trichloroethene + reduced acceptor
-
Dehalobacter restrictus cis-1,2-dichloroethene + chloride + acceptor
-
?

Subunits

EC Number Subunits Comment Organism
1.21.99.5 monomer 1 * 60000, SDS-PAGE Dehalobacter restrictus

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.99.5 vitamin B12
-
Dehalobacter restrictus