Literature summary extracted from

Muranova, T.A.; Ruzheinikov, S.N.; Sedelnikova, S.E.; Baker, P.J.; Pasquo, A.; Galkin, A.; Esaki, N.; Ohshima, T.; Soda, K.; Rice, D.W.
Crystallization and preliminary X-ray analysis of substrate complexes of leucine dehydrogenase from Thermoactinomyces intermedius (2002), Acta Crystallogr. Sect. D, 58, 1059-1062.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.4.1.9	crystals of the binary complex with 4-methyl-2-oxopentanoate, hanging-drop vapour-diffusion method using PEG 4000 as precipitant	Thermoactinomyces intermedius

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.1.9	Lysinibacillus sphaericus	-	-	-
1.4.1.9	Thermoactinomyces intermedius	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.4.1.9	L-Leu + H2O + NAD+	-	Lysinibacillus sphaericus	4-methyl-2-oxopentanoate + NH3 + NADH	-	r
1.4.1.9	L-Leu + H2O + NAD+	-	Thermoactinomyces intermedius	4-methyl-2-oxopentanoate + NH3 + NADH	-	r

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.4.1.9	NAD+	-	Lysinibacillus sphaericus
1.4.1.9	NAD+	-	Thermoactinomyces intermedius
1.4.1.9	NADH	-	Lysinibacillus sphaericus
1.4.1.9	NADH	-	Thermoactinomyces intermedius

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Release 2023.1 (January 2023)