EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.9 | - |
Thermoactinomyces intermedius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.9 | 0.042 | - |
NADH | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 0.36 | - |
NAD+ | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 0.4 | - |
L-Ile | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 0.63 | - |
4-methyl-2-oxopentanoate | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 0.91 | - |
2-oxopentanoate | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 1.4 | - |
2-Oxohexanoate | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 2 | - |
L-Leu | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 2.2 | - |
2-oxo-3-methylpentanoate | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 2.4 | - |
L-Val | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 2.7 | - |
4-methyl-2-oxo-5,5,5-trifluoropentanoate | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 2.8 | - |
NADP+ | mutant enzyme D203A/I204R | Thermoactinomyces intermedius | |
1.4.1.9 | 4.4 | - |
3-methyl-2-oxobutanoate | - |
Thermoactinomyces intermedius | |
1.4.1.9 | 7.2 | - |
2-oxobutanoate | - |
Thermoactinomyces intermedius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 42000 | - |
4 * 42000, SDS-PAGE | Thermoactinomyces intermedius |
1.4.1.9 | 340000 | - |
gel filtration | Thermoactinomyces intermedius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.9 | Thermoactinomyces intermedius | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.9 | - |
Thermoactinomyces intermedius |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 102 | - |
- |
Thermoactinomyces intermedius |
1.4.1.9 | 122 | - |
recombinant enzyme | Thermoactinomyces intermedius |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.9 | 4°C, stable for over two years | Thermoactinomyces intermedius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.9 | 2-oxohexanoate + NH3 + NADH + H+ | 46% activity compared to 2-oxoisocaproate | Thermoactinomyces intermedius | L-norleucine + H2O + NAD+ | - |
r | |
1.4.1.9 | 4-methyl-2-oxo-5,5,5-trifluoropentanoate + NH3 + NADH | - |
Thermoactinomyces intermedius | 2-amino-4-methyl-5,5,5-trifluoropentanoate + NAD+ + H2O | - |
? | |
1.4.1.9 | L-2-aminobutanoate + H2O + NAD+ | - |
Thermoactinomyces intermedius | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
1.4.1.9 | L-2-aminobutanoate + H2O + NAD+ | - |
Thermoactinomyces intermedius | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.9 | L-Ile + H2O + NAD+ | - |
Thermoactinomyces intermedius | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-Leu + H2O + NAD+ | - |
Thermoactinomyces intermedius | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-Val + H2O + NAD+ | - |
Thermoactinomyces intermedius | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.9 | octamer | 4 * 42000, SDS-PAGE | Thermoactinomyces intermedius |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 65 | - |
10 min, retains its full activity | Thermoactinomyces intermedius |
1.4.1.9 | 70 | - |
40 min, 3 M NaCl, less than 10% loss of activity | Thermoactinomyces intermedius |
1.4.1.9 | 75 | - |
10 min, about 75% loss of activity | Thermoactinomyces intermedius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 9 | - |
reductive amination of 3-methyl-2-oxobutanoate | Thermoactinomyces intermedius |
1.4.1.9 | 9.3 | - |
reductive amination of 2-oxo-3-methylpentanoate | Thermoactinomyces intermedius |
1.4.1.9 | 9.6 | - |
reductive amination of 4-methyl-2-oxopentanoate | Thermoactinomyces intermedius |
1.4.1.9 | 10 | - |
deamination of L-Val | Thermoactinomyces intermedius |
1.4.1.9 | 10.5 | - |
deamination of L-Leu | Thermoactinomyces intermedius |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 6 | 10.9 | 50°C, 5 min, stable | Thermoactinomyces intermedius |
1.4.1.9 | 7.5 | - |
70°C, 5 min, most stable at pH 7.5 | Thermoactinomyces intermedius |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | NAD+ | - |
Thermoactinomyces intermedius | |
1.4.1.9 | NADH | - |
Thermoactinomyces intermedius |