EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.9 | cloned into Escherichia coli JM 109 with a vector plasmid pUC18 | Bacillus licheniformis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | NADH | noncompetitive inhibition with respect to L-Leu | Bacillus licheniformis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.9 | 1 | - |
4-methyl-2-oxopentanoate | - |
Bacillus licheniformis | |
1.4.1.9 | 2.1 | - |
L-Leu | - |
Bacillus licheniformis | |
1.4.1.9 | 2.4 | - |
2-oxopentanoate | - |
Bacillus licheniformis | |
1.4.1.9 | 3.3 | - |
L-Ile | - |
Bacillus licheniformis | |
1.4.1.9 | 3.8 | - |
3-methyl-2-oxobutanoate | - |
Bacillus licheniformis | |
1.4.1.9 | 12.5 | - |
L-Val | - |
Bacillus licheniformis | |
1.4.1.9 | 330 | - |
NH3 | - |
Bacillus licheniformis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 44000 | - |
8 * 44000, SDS-PAGE | Bacillus licheniformis |
1.4.1.9 | 360000 | - |
gel filtration | Bacillus licheniformis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.9 | Bacillus licheniformis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.9 | - |
Bacillus licheniformis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.9 | 2-oxopentanoate + NH3 + NADH + H+ | - |
Bacillus licheniformis | L-norvaline + H2O + NAD+ | - |
r | |
1.4.1.9 | L-2-aminobutanoate + H2O + NAD+ | 32% of the activity with L-Leu | Bacillus licheniformis | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.9 | L-Ala + H2O + NAD+ | 10% of the activity with L-Leu | Bacillus licheniformis | 2-oxopropanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Ile + H2O + NAD+ | 72% of the activity with L-Leu | Bacillus licheniformis | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-Leu + H2O + NAD+ | - |
Bacillus licheniformis | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-norleucine + H2O + NAD+ | 7% of the activity with L-Leu | Bacillus licheniformis | 2-oxohexanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-S-methylcysteine + H2O + NAD+ | 19% of the activity with L-Leu | Bacillus licheniformis | 3-methylthio-2-oxopropionate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Val + H2O + NAD+ | 59% of the activity with L-Leu | Bacillus licheniformis | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.9 | octamer | 8 * 44000, SDS-PAGE | Bacillus licheniformis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 30 | - |
10 mM potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 1 mM EDTA, 1.0 M NaCl, stable for more than 6 months | Bacillus licheniformis |
1.4.1.9 | 65 | - |
1 h, in presence of 2.5 M NaCl, stable | Bacillus licheniformis |
1.4.1.9 | 70 | - |
1 h, complete loss of activity | Bacillus licheniformis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 8.6 | - |
reductive amination of 3-methyl-2-oxobutanoate and 2-oxopentanoate | Bacillus licheniformis |
1.4.1.9 | 9.3 | - |
reductive amination of 4-methyl-2-oxopentanoate | Bacillus licheniformis |
1.4.1.9 | 10.3 | - |
oxidative deamination of L-Leu in presence of 1.0 M NaCl | Bacillus licheniformis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | additional information | no activity with NADPH | Bacillus licheniformis | |
1.4.1.9 | additional information | no activity with NADP+ | Bacillus licheniformis | |
1.4.1.9 | NAD+ | - |
Bacillus licheniformis | |
1.4.1.9 | NADH | - |
Bacillus licheniformis |