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Literature summary extracted from

  • Nagata, S.; Bakthavatsalam, S.; Galkin, A.G.; Asada, H.; Sakai, S.; Esaki, N.; Soda, K.; Ohshima, T.; Nagasaki, S.; Misono, H.
    Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9 (1995), Appl. Microbiol. Biotechnol., 44, 432-438.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.9 cloned into Escherichia coli JM 109 with a vector plasmid pUC18 Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 NADH noncompetitive inhibition with respect to L-Leu Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 1
-
4-methyl-2-oxopentanoate
-
Bacillus licheniformis
1.4.1.9 2.1
-
L-Leu
-
Bacillus licheniformis
1.4.1.9 2.4
-
2-oxopentanoate
-
Bacillus licheniformis
1.4.1.9 3.3
-
L-Ile
-
Bacillus licheniformis
1.4.1.9 3.8
-
3-methyl-2-oxobutanoate
-
Bacillus licheniformis
1.4.1.9 12.5
-
L-Val
-
Bacillus licheniformis
1.4.1.9 330
-
NH3
-
Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 44000
-
8 * 44000, SDS-PAGE Bacillus licheniformis
1.4.1.9 360000
-
gel filtration Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Bacillus licheniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 2-oxopentanoate + NH3 + NADH + H+
-
Bacillus licheniformis L-norvaline + H2O + NAD+
-
r
1.4.1.9 L-2-aminobutanoate + H2O + NAD+ 32% of the activity with L-Leu Bacillus licheniformis 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.9 L-Ala + H2O + NAD+ 10% of the activity with L-Leu Bacillus licheniformis 2-oxopropanoate + NH3 + NADH
-
?
1.4.1.9 L-Ile + H2O + NAD+ 72% of the activity with L-Leu Bacillus licheniformis 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Bacillus licheniformis 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-norleucine + H2O + NAD+ 7% of the activity with L-Leu Bacillus licheniformis 2-oxohexanoate + NH3 + NADH
-
r
1.4.1.9 L-S-methylcysteine + H2O + NAD+ 19% of the activity with L-Leu Bacillus licheniformis 3-methylthio-2-oxopropionate + NH3 + NADH
-
?
1.4.1.9 L-Val + H2O + NAD+ 59% of the activity with L-Leu Bacillus licheniformis 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.9 octamer 8 * 44000, SDS-PAGE Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 30
-
10 mM potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 1 mM EDTA, 1.0 M NaCl, stable for more than 6 months Bacillus licheniformis
1.4.1.9 65
-
1 h, in presence of 2.5 M NaCl, stable Bacillus licheniformis
1.4.1.9 70
-
1 h, complete loss of activity Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 8.6
-
reductive amination of 3-methyl-2-oxobutanoate and 2-oxopentanoate Bacillus licheniformis
1.4.1.9 9.3
-
reductive amination of 4-methyl-2-oxopentanoate Bacillus licheniformis
1.4.1.9 10.3
-
oxidative deamination of L-Leu in presence of 1.0 M NaCl Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 additional information no activity with NADPH Bacillus licheniformis
1.4.1.9 additional information no activity with NADP+ Bacillus licheniformis
1.4.1.9 NAD+
-
Bacillus licheniformis
1.4.1.9 NADH
-
Bacillus licheniformis