Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nagata, S.; Misono, H.; Nagasaki, S.; Esaki, N.; Tanaka, H.; Soda, K.
    Gene cloning, purification, and characterization of the highly thermostable leucine dehydrogenase of bacillus sp. (1990), J. Ferment. Bioeng., 69, 199-203.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.9 expression in Escherichia coli Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 1.2
-
 NAD+
-
 Bacillus sp. (in: Bacteria)
1.4.1.9 2.8
-
 L-Leu
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 47000
-
 6 * 47000, SDS-PAGE Bacillus sp. (in: Bacteria)
1.4.1.9 280000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Bacillus sp. (in: Bacteria)
-
 DSM 730
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 110
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-Ile + H2O + NAD+
-
 Bacillus sp. (in: Bacteria) 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.9 L-Leu + H2O + NAD+
-
 Bacillus sp. (in: Bacteria) 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.9 L-Val + H2O + NAD+
-
 Bacillus sp. (in: Bacteria) 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.9 hexamer 6 * 47000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 55
-
 pH 5.4-10.3, 10 min, stable Bacillus sp. (in: Bacteria)
1.4.1.9 80
-
 10 min, stable Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 9.6
-
 reductive amination of 4-methyl-2-oxopentanoate Bacillus sp. (in: Bacteria)
1.4.1.9 11.3
-
 oxidative deamination of L-Leu Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.9 5.4 10.3 55°C, 10 min, stable Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 additional information no activity with NADPH Bacillus sp. (in: Bacteria)
1.4.1.9 additional information no activity with NADP+ Bacillus sp. (in: Bacteria)
1.4.1.9 NAD+
-
 Bacillus sp. (in: Bacteria)
1.4.1.9 NADH
-
 Bacillus sp. (in: Bacteria)