Literature summary extracted from

Kiba, N.; Hori, S.; Furusawa, M.
Flow-injection determination of branched-chain L-amino acids with immobilized leucine dehydrogenase (1989), Anal. Chim. Acta, 218, 161-166.

No PubMed abstract available

Application

EC Number	Application	Comment	Organism
1.4.1.9	analysis	flow-injection determination of branched-chain L-amino acids with immobilized leucine dehydrogenase	Bacillus sp. (in: Bacteria)

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.4.1.9	Co2+	-	Bacillus sp. (in: Bacteria)
1.4.1.9	Cu2+	-	Bacillus sp. (in: Bacteria)

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.1.9	Bacillus cereus	-	-	-
1.4.1.9	Bacillus sp. (in: Bacteria)	-	-	-

Storage Stability

EC Number	Storage Stability	Organism
1.4.1.9	4°C, 5 mM tetrasodium EDTA solution in phosphate buffer, 0.1 M, pH 7.0, stable for at least 2 months	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.4.1.9	L-Leu + H2O + NAD+	-	Bacillus cereus	4-methyl-2-oxopentanoate + NH3 + NADH	-	r
1.4.1.9	L-Leu + H2O + NAD+	-	Bacillus sp. (in: Bacteria)	4-methyl-2-oxopentanoate + NH3 + NADH	-	r

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.4.1.9	50	-	-	Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
1.4.1.9	30	52	30°C: about 45% of maximal activity, 48-52°C: maximal activity	Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.4.1.9	52	-	denaturation above	Bacillus sp. (in: Bacteria)

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.4.1.9	11	-	oxidative deamination of L-Leu	Bacillus sp. (in: Bacteria)

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
1.4.1.9	10	11.5	pH 10.0: about 40% of maximal activity, pH 11.5: about 80% of maximal activity, oxidative deamination of L-Leu	Bacillus sp. (in: Bacteria)

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.4.1.9	NAD+	-	Bacillus cereus
1.4.1.9	NAD+	-	Bacillus sp. (in: Bacteria)
1.4.1.9	NADH	-	Bacillus cereus
1.4.1.9	NADH	-	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)