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Literature summary extracted from

  • Esaki, N.; Shimoi, H.; Yang, Y.S.; Tanaka, H.; Soda, K.
    Enantioselective synthesis of L-selenomethionine with leucine dehydrogenase (1989), Biotechnol. Appl. Biochem., 11, 312-317.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.4.1.9 synthesis synthesis of L-selenomethionine from 2-oxo-4-methylselenobutanoate Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 13
-
 2-oxo-4-methylselenobutanoate
-
 Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Geobacillus stearothermophilus
-
 recombinant enzyme expressed in Escherichia coli JM109 cells carrying the vector plasmid pICD212
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 2-oxo-4-methylselenobutyrate + NH3 + NADH
-
 Geobacillus stearothermophilus L-selenomethionine + H2O + NAD+
-
 ?
1.4.1.9 L-Leu + H2O + NAD+
-
 Geobacillus stearothermophilus 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 9.7
-
 reductive amination of 2-oxo-4-methylselenobutanoate Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.9 8.7 10.7 pH 8.7: about 65% of maximal activity, pH 10.7: about 45% of maximal activity, reductive amination of 2-oxo-4-methylselenobutanoate Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NAD+
-
 Geobacillus stearothermophilus
1.4.1.9 NADH
-
 Geobacillus stearothermophilus