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Literature summary extracted from

  • Ohshima, T.; Misono, H.; Soda, K.
    Properties of crystalline leucine dehydrogenase from Bacillus sphaericus (1978), J. Biol. Chem., 253, 5719-5725.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.9 crystallized by addition of ammonium sulfate Lysinibacillus sphaericus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 Co2+ CoCl2 Lysinibacillus sphaericus
1.4.1.9 Cu2+ CuSO4 Lysinibacillus sphaericus
1.4.1.9 D-2-aminobutanoate
-
Lysinibacillus sphaericus
1.4.1.9 D-alloisoleucine
-
Lysinibacillus sphaericus
1.4.1.9 D-Leu
-
Lysinibacillus sphaericus
1.4.1.9 D-norleucine
-
Lysinibacillus sphaericus
1.4.1.9 D-Norvaline
-
Lysinibacillus sphaericus
1.4.1.9 D-Val
-
Lysinibacillus sphaericus
1.4.1.9 HgCl2
-
Bacillus cereus
1.4.1.9 HgCl2
-
Lysinibacillus sphaericus
1.4.1.9 MgCl2
-
Lysinibacillus sphaericus
1.4.1.9 Na2S
-
Lysinibacillus sphaericus
1.4.1.9 NAD+ product inhibition Lysinibacillus sphaericus
1.4.1.9 PCMB
-
Bacillus cereus
1.4.1.9 PCMB
-
Lysinibacillus sphaericus
1.4.1.9 pyridoxal 5'-phosphate
-
Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.31
-
4-methyl-2-oxopentanoate
-
Lysinibacillus sphaericus
1.4.1.9 0.39
-
NAD+
-
Lysinibacillus sphaericus
1.4.1.9 0.77
-
3-acetylpyridine-NAD+
-
Lysinibacillus sphaericus
1.4.1.9 1
-
L-Leu
-
Lysinibacillus sphaericus
1.4.1.9 1.4
-
3-methyl-2-oxobutanoate
-
Lysinibacillus sphaericus
1.4.1.9 1.7
-
L-Val
-
Lysinibacillus sphaericus
1.4.1.9 1.7
-
2-oxopentanoate
-
Lysinibacillus sphaericus
1.4.1.9 1.8
-
L-Ile
-
Lysinibacillus sphaericus
1.4.1.9 2.41
-
3-acetylpyridine-deamino-NAD+
-
Lysinibacillus sphaericus
1.4.1.9 3.5
-
L-norvaline
-
Lysinibacillus sphaericus
1.4.1.9 6.3
-
L-norleucine
-
Lysinibacillus sphaericus
1.4.1.9 7
-
2-Oxohexanoate
-
Lysinibacillus sphaericus
1.4.1.9 7.7
-
2-oxobutanoate
-
Lysinibacillus sphaericus
1.4.1.9 10
-
L-2-aminobutanoate
-
Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 41000
-
6 * 41000, SDS-PAGE Lysinibacillus sphaericus
1.4.1.9 245000
-
equilibrium sedimentation Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Bacillus cereus
-
-
-
1.4.1.9 Bacillus pumilus
-
var. flavus
-
1.4.1.9 Bacillus subtilis
-
-
-
1.4.1.9 Brevibacillus brevis
-
-
-
1.4.1.9 Lysinibacillus sphaericus
-
-
-
1.4.1.9 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 42.3
-
-
Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-2-aminobutanoate + H2O + NAD+
-
Bacillus subtilis 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.9 L-2-aminobutanoate + H2O + NAD+
-
Bacillus cereus 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.9 L-2-aminobutanoate + H2O + NAD+ 41% of the activity with L-Leu Lysinibacillus sphaericus 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.9 L-2-aminobutanoate + H2O + NAD+ 14% of the activity with L-Leu Lysinibacillus sphaericus 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.9 L-Ile + H2O + NAD+
-
Bacillus subtilis 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Ile + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Ile + H2O + NAD+ 58% of the activity with L-Leu Lysinibacillus sphaericus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + 3-acetylpyridine-deamino-NAD+ as effective as NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-deamino-NADH
-
?
1.4.1.9 L-Leu + H2O + 3-acetylpyridine-NAD+ 166% of the activity with NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-NADH
-
?
1.4.1.9 L-Leu + H2O + 3-pyridinealdehyde-NAD+ 19% of the activity with NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + 3-pyridinealdehyde-NADH
-
?
1.4.1.9 L-Leu + H2O + deamino-NAD+ 81% of the activity with NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + deamino-NADH
-
?
1.4.1.9 L-Leu + H2O + NAD+
-
Brevibacillus brevis 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Bacillus subtilis 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Priestia megaterium 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Bacillus cereus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Bacillus pumilus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + thionicotinamide-NAD+ 21% of the activity with NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + thionicotinamide-NADH
-
?
1.4.1.9 L-norleucine + H2O + NAD+
-
Bacillus subtilis 2-oxohexanoate + NH3 + NADH
-
?
1.4.1.9 L-norleucine + H2O + NAD+
-
Bacillus cereus 2-oxohexanoate + NH3 + NADH
-
r
1.4.1.9 L-norleucine + H2O + NAD+ 74% of the activity with L-Leu Lysinibacillus sphaericus 2-oxohexanoate + NH3 + NADH
-
r
1.4.1.9 L-tert-Leu + H2O + NAD+
-
Lysinibacillus sphaericus 3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
?
1.4.1.9 L-Val + H2O + NAD+
-
Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
1.4.1.9 L-Val + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
1.4.1.9 L-Val + H2O + NAD+ 74% of the activity with L-Leu Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
1.4.1.9 additional information the enzyme is B-stereospecific Lysinibacillus sphaericus ?
-
?
1.4.1.9 additional information reductive amination proceeds through a sequential ordered ternary-binary mechanism Lysinibacillus sphaericus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.4.1.9 hexamer 6 * 41000, SDS-PAGE Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NAD+
-
Brevibacillus brevis
1.4.1.9 NAD+
-
Bacillus subtilis
1.4.1.9 NAD+
-
Priestia megaterium
1.4.1.9 NAD+
-
Lysinibacillus sphaericus
1.4.1.9 NAD+
-
Bacillus cereus
1.4.1.9 NAD+
-
Bacillus pumilus
1.4.1.9 NADH
-
Brevibacillus brevis
1.4.1.9 NADH
-
Bacillus subtilis
1.4.1.9 NADH
-
Priestia megaterium
1.4.1.9 NADH
-
Lysinibacillus sphaericus
1.4.1.9 NADH
-
Bacillus cereus
1.4.1.9 NADH
-
Bacillus pumilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.9 2.2
-
D-Leu inhibition of deamination of D-Leu Lysinibacillus sphaericus
1.4.1.9 3
-
D-Norvaline inhibition of deamination of D-Leu Lysinibacillus sphaericus
1.4.1.9 7.9
-
D-alloisoleucine inhibition of deamination of D-Leu Lysinibacillus sphaericus
1.4.1.9 9.4
-
D-2-aminobutanoate inhibition of deamination of D-Leu Lysinibacillus sphaericus
1.4.1.9 10.8
-
D-Val inhibition of deamination of D-Leu Lysinibacillus sphaericus
1.4.1.9 52.6
-
D-norleucine inhibition of deamination of D-Leu Lysinibacillus sphaericus