EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | HgCl2 | - |
[Bacillus] caldolyticus | |
1.4.1.9 | p-mercuribenzoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | pyridoxal 5'-phosphate | - |
[Bacillus] caldolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.9 | 0.036 | - |
NADH | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 0.18 | - |
NAD+ | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 0.7 | - |
4-methyl-2-oxopentanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 0.7 | - |
L-Ile | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 0.81 | - |
2-oxopentanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 1.1 | - |
L-Leu | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 1.8 | - |
L-tert-Leu | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 1.9 | - |
2-oxo-3-methylpentanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 2 | - |
L-Val | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 2.1 | - |
L-norleucine | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 2.3 | - |
L-norvaline | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 2.4 | - |
3-methyl-2-oxobutanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 2.7 | - |
2-Oxohexanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 6.9 | - |
2-oxobutanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 8.1 | - |
4-(methylsulfanyl)-2-oxobutanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 8.9 | - |
L-2-aminobutanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 19 | - |
2-oxo-3,3-dimethylbutanoate | - |
[Bacillus] caldolyticus | |
1.4.1.9 | 23 | - |
L-Met | - |
[Bacillus] caldolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 39000 | - |
8 * 39000, SDS-PAGE | [Bacillus] caldolyticus |
1.4.1.9 | 313000 | - |
non-denaturing PAGE | [Bacillus] caldolyticus |
1.4.1.9 | 325000 | - |
gel filtration | [Bacillus] caldolyticus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.4.1.9 | 2-propanol | 2 M, 4°C, 5% loss of activity after 2 months | [Bacillus] caldolyticus |
1.4.1.9 | acetonitrile | 2 M, 4°C, 22% loss of activity after 3 d | [Bacillus] caldolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.9 | [Bacillus] caldolyticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.9 | - |
[Bacillus] caldolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | additional information | - |
- |
[Bacillus] caldolyticus |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.9 | 4°C, 50 mM potassium phosphate, 1 mM dithioerythritol, pH 7.5, 15% loss of activity after 2 months | [Bacillus] caldolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.9 | 2-oxo-3,3-dimethylbutanoate + NH3 + NADH | - |
[Bacillus] caldolyticus | L-2-amino-3,3-dimethylbutanoate + NAD+ + H2O | - |
? | |
1.4.1.9 | 4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+ | - |
[Bacillus] caldolyticus | L-methionine + H2O + NAD+ | - |
r | |
1.4.1.9 | L-2-aminobutanoate + H2O + NAD+ | - |
[Bacillus] caldolyticus | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.9 | L-Ile + H2O + NAD+ | - |
[Bacillus] caldolyticus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-Leu + H2O + NAD+ | - |
[Bacillus] caldolyticus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-Met + H2O + NAD+ | - |
[Bacillus] caldolyticus | 4-methylthio-2-oxobutyrate + NH3 + NADH + H+ | - |
? | |
1.4.1.9 | L-norleucine + H2O + NAD+ | - |
[Bacillus] caldolyticus | 2-oxohexanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-norvaline + H2O + NAD+ | - |
[Bacillus] caldolyticus | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.9 | L-tert-Leu + H2O + NAD+ | - |
[Bacillus] caldolyticus | 3,3-dimethyl-2-oxobutanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Val + H2O + NAD+ | - |
[Bacillus] caldolyticus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.9 | octamer | 8 * 39000, SDS-PAGE | [Bacillus] caldolyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 80 | - |
pH 7.5, 30 min, 50% loss of activity | [Bacillus] caldolyticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 6.5 | 10.5 | 30 min, 50% loss of activity | [Bacillus] caldolyticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | NAD+ | - |
[Bacillus] caldolyticus | |
1.4.1.9 | NADH | - |
[Bacillus] caldolyticus |