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Literature summary extracted from

  • Kaerst, U.; Schuette, H.; Baydoun, H.; Tsai, H.
    Purification and characterization of leucine dehydrogenase from the thermophile Bacillus caldolyticus (1989), J. Gen. Microbiol., 135, 1305-1313.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 HgCl2
-
 [Bacillus] caldolyticus
1.4.1.9 p-mercuribenzoate
-
 [Bacillus] caldolyticus
1.4.1.9 pyridoxal 5'-phosphate
-
 [Bacillus] caldolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.036
-
 NADH
-
 [Bacillus] caldolyticus
1.4.1.9 0.18
-
 NAD+
-
 [Bacillus] caldolyticus
1.4.1.9 0.7
-
 4-methyl-2-oxopentanoate
-
 [Bacillus] caldolyticus
1.4.1.9 0.7
-
 L-Ile
-
 [Bacillus] caldolyticus
1.4.1.9 0.81
-
 2-oxopentanoate
-
 [Bacillus] caldolyticus
1.4.1.9 1.1
-
 L-Leu
-
 [Bacillus] caldolyticus
1.4.1.9 1.8
-
 L-tert-Leu
-
 [Bacillus] caldolyticus
1.4.1.9 1.9
-
 2-oxo-3-methylpentanoate
-
 [Bacillus] caldolyticus
1.4.1.9 2
-
 L-Val
-
 [Bacillus] caldolyticus
1.4.1.9 2.1
-
 L-norleucine
-
 [Bacillus] caldolyticus
1.4.1.9 2.3
-
 L-norvaline
-
 [Bacillus] caldolyticus
1.4.1.9 2.4
-
 3-methyl-2-oxobutanoate
-
 [Bacillus] caldolyticus
1.4.1.9 2.7
-
 2-Oxohexanoate
-
 [Bacillus] caldolyticus
1.4.1.9 6.9
-
 2-oxobutanoate
-
 [Bacillus] caldolyticus
1.4.1.9 8.1
-
 4-(methylsulfanyl)-2-oxobutanoate
-
 [Bacillus] caldolyticus
1.4.1.9 8.9
-
 L-2-aminobutanoate
-
 [Bacillus] caldolyticus
1.4.1.9 19
-
 2-oxo-3,3-dimethylbutanoate
-
 [Bacillus] caldolyticus
1.4.1.9 23
-
 L-Met
-
 [Bacillus] caldolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 39000
-
 8 * 39000, SDS-PAGE [Bacillus] caldolyticus
1.4.1.9 313000
-
 non-denaturing PAGE [Bacillus] caldolyticus
1.4.1.9 325000
-
 gel filtration [Bacillus] caldolyticus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.4.1.9 2-propanol 2 M, 4°C, 5% loss of activity after 2 months [Bacillus] caldolyticus
1.4.1.9 acetonitrile 2 M, 4°C, 22% loss of activity after 3 d [Bacillus] caldolyticus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 [Bacillus] caldolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
 [Bacillus] caldolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 additional information
-
-
 [Bacillus] caldolyticus

Storage Stability

EC Number Storage Stability Organism
1.4.1.9 4°C, 50 mM potassium phosphate, 1 mM dithioerythritol, pH 7.5, 15% loss of activity after 2 months [Bacillus] caldolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 2-oxo-3,3-dimethylbutanoate + NH3 + NADH
-
 [Bacillus] caldolyticus L-2-amino-3,3-dimethylbutanoate + NAD+ + H2O
-
 ?
1.4.1.9 4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+
-
 [Bacillus] caldolyticus L-methionine + H2O + NAD+
-
 r
1.4.1.9 L-2-aminobutanoate + H2O + NAD+
-
 [Bacillus] caldolyticus 2-oxobutanoate + NH3 + NADH + H+
-
 r
1.4.1.9 L-Ile + H2O + NAD+
-
 [Bacillus] caldolyticus 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.9 L-Leu + H2O + NAD+
-
 [Bacillus] caldolyticus 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.9 L-Met + H2O + NAD+
-
 [Bacillus] caldolyticus 4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
 ?
1.4.1.9 L-norleucine + H2O + NAD+
-
 [Bacillus] caldolyticus 2-oxohexanoate + NH3 + NADH
-
 r
1.4.1.9 L-norvaline + H2O + NAD+
-
 [Bacillus] caldolyticus 2-oxopentanoate + NH3 + NADH + H+
-
 r
1.4.1.9 L-tert-Leu + H2O + NAD+
-
 [Bacillus] caldolyticus 3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.9 L-Val + H2O + NAD+
-
 [Bacillus] caldolyticus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.9 octamer 8 * 39000, SDS-PAGE [Bacillus] caldolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 80
-
 pH 7.5, 30 min, 50% loss of activity [Bacillus] caldolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.9 6.5 10.5 30 min, 50% loss of activity [Bacillus] caldolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NAD+
-
 [Bacillus] caldolyticus
1.4.1.9 NADH
-
 [Bacillus] caldolyticus