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Literature summary extracted from

  • Ohshima, T.; Nagata, S.; Soda, K.
    Purification and characterization of thermostable leucine dehydrogenase from Bacillus stearothermophilus (1985), Arch. Microbiol., 141, 407-411.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 Cu(CH3COO)2 1 mM, 20% inhibition Geobacillus stearothermophilus
1.4.1.9 Cu2+ 1 mM CuCl2, 22% inhibition Geobacillus stearothermophilus
1.4.1.9 HgCl2 0.01 M, 65% inhibition Geobacillus stearothermophilus
1.4.1.9 Pb(CH3COO)2 1 mM, 20% inhibition Geobacillus stearothermophilus
1.4.1.9 PCMB 0.01 M, 50% inhibition Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 49000
-
6 * 49000, SDS-PAGE Geobacillus stearothermophilus
1.4.1.9 300000
-
gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 120
-
-
Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
1.4.1.9 4°C, stable for at least 1 month Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-Ile + H2O + NAD+ 73% of the activity with L-Leu Geobacillus stearothermophilus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Geobacillus stearothermophilus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Met + H2O + NAD+ no activity Geobacillus stearothermophilus 4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
?
1.4.1.9 L-Val + H2O + NAD+ 98% of the activity with L-Leu Geobacillus stearothermophilus 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.9 hexamer 6 * 49000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.9 68 70 oxidative amination of L-Leu Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.9 50 80 50°C: 37% of maximal activity, 80°C: 55% of maximal activity, oxidative amination of L-Leu Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 70
-
20 min, enzyme retains full activity Geobacillus stearothermophilus
1.4.1.9 80
-
5 min, substantial loss of activity Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 8.8
-
reductive amination of 3-methyl-2-oxobutanoate Geobacillus stearothermophilus
1.4.1.9 9.7
-
reductive amination of 4-methyl-2-oxopentanoate Geobacillus stearothermophilus
1.4.1.9 11
-
oxidative deamination of L-Leu Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.9 5.5 10 55°C, 5 min, stable Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 additional information no activity with NADPH Geobacillus stearothermophilus
1.4.1.9 additional information no activity with NADP+ Geobacillus stearothermophilus
1.4.1.9 NAD+
-
Geobacillus stearothermophilus
1.4.1.9 NADH
-
Geobacillus stearothermophilus