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Literature summary extracted from

  • Schuette, H.; Hummel, W.; Tsai, H.; Kula, M.R.
    L-Leucine dehydrogenase from Bacillus cereus (1985), Appl. Microbiol. Biotechnol., 22, 306-317.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.9
-
Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 HgCl2 1 mM, 92% inhibition Bacillus cereus
1.4.1.9 KCN 1 mM, 43% inhibition Bacillus cereus
1.4.1.9 p-mercuribenzoate 1 mM, 75% inhibition Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.034
-
NADH
-
Bacillus cereus
1.4.1.9 0.34
-
NAD+
-
Bacillus cereus
1.4.1.9 0.4
-
2-oxopentanoate
-
Bacillus cereus
1.4.1.9 0.45
-
4-methyl-2-oxopentanoate
-
Bacillus cereus
1.4.1.9 0.9
-
2-oxo-3-methylpentanoate
-
Bacillus cereus
1.4.1.9 1
-
L-Ile
-
Bacillus cereus
1.4.1.9 1.2
-
2-Oxohexanoate
-
Bacillus cereus
1.4.1.9 1.5
-
2-oxobutanoate
-
Bacillus cereus
1.4.1.9 1.5
-
L-Leu
-
Bacillus cereus
1.4.1.9 1.5
-
L-norleucine
-
Bacillus cereus
1.4.1.9 2.1
-
3-methyl-2-oxobutanoate
-
Bacillus cereus
1.4.1.9 2.1
-
3-methylthio-2-oxobutanoate
-
Bacillus cereus
1.4.1.9 2.5
-
L-Val
-
Bacillus cereus
1.4.1.9 2.9
-
L-norvaline
-
Bacillus cereus
1.4.1.9 22
-
L-2-aminobutanoate
-
Bacillus cereus
1.4.1.9 23
-
L-Met
-
Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 39000
-
8 * 39000, SDS-PAGE Bacillus cereus
1.4.1.9 310000
-
gel filtration, equilibrium sedimenation Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 57
-
-
Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-2-aminobutanoate + H2O + NAD+
-
Bacillus cereus 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.9 L-Ile + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Bacillus cereus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Met + H2O + NAD+
-
Bacillus cereus 4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
r
1.4.1.9 L-norleucine + H2O + NAD+
-
Bacillus cereus 2-oxohexanoate + NH3 + NADH
-
r
1.4.1.9 L-norvaline + H2O + NAD+
-
Bacillus cereus 2-oxopentanoate + NH3 + NADH + H+
-
r
1.4.1.9 L-Val + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.9 octamer 8 * 39000, SDS-PAGE Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 8.5
-
reductive amination of 2-oxopentanoate and 3-methyl-2-oxobutanoate Bacillus cereus
1.4.1.9 9 9.5 reductive amination of 4-methyl-2-oxopentanoate, 2-oxopentanoate and 2-oxomethionine Bacillus cereus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.9 5.6 9.8 25°C, 24 h, maximal loss of 12% of the activity Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 additional information no activity with NADPH Bacillus cereus
1.4.1.9 additional information no activity with NADP+ Bacillus cereus
1.4.1.9 NAD+
-
Bacillus cereus
1.4.1.9 NADH
-
Bacillus cereus