EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.1.9 | - |
Bacillus cereus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | HgCl2 | 1 mM, 92% inhibition | Bacillus cereus | |
1.4.1.9 | KCN | 1 mM, 43% inhibition | Bacillus cereus | |
1.4.1.9 | p-mercuribenzoate | 1 mM, 75% inhibition | Bacillus cereus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.9 | 0.034 | - |
NADH | - |
Bacillus cereus | |
1.4.1.9 | 0.34 | - |
NAD+ | - |
Bacillus cereus | |
1.4.1.9 | 0.4 | - |
2-oxopentanoate | - |
Bacillus cereus | |
1.4.1.9 | 0.45 | - |
4-methyl-2-oxopentanoate | - |
Bacillus cereus | |
1.4.1.9 | 0.9 | - |
2-oxo-3-methylpentanoate | - |
Bacillus cereus | |
1.4.1.9 | 1 | - |
L-Ile | - |
Bacillus cereus | |
1.4.1.9 | 1.2 | - |
2-Oxohexanoate | - |
Bacillus cereus | |
1.4.1.9 | 1.5 | - |
2-oxobutanoate | - |
Bacillus cereus | |
1.4.1.9 | 1.5 | - |
L-Leu | - |
Bacillus cereus | |
1.4.1.9 | 1.5 | - |
L-norleucine | - |
Bacillus cereus | |
1.4.1.9 | 2.1 | - |
3-methyl-2-oxobutanoate | - |
Bacillus cereus | |
1.4.1.9 | 2.1 | - |
3-methylthio-2-oxobutanoate | - |
Bacillus cereus | |
1.4.1.9 | 2.5 | - |
L-Val | - |
Bacillus cereus | |
1.4.1.9 | 2.9 | - |
L-norvaline | - |
Bacillus cereus | |
1.4.1.9 | 22 | - |
L-2-aminobutanoate | - |
Bacillus cereus | |
1.4.1.9 | 23 | - |
L-Met | - |
Bacillus cereus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 39000 | - |
8 * 39000, SDS-PAGE | Bacillus cereus |
1.4.1.9 | 310000 | - |
gel filtration, equilibrium sedimenation | Bacillus cereus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.9 | Bacillus cereus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.9 | - |
Bacillus cereus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 57 | - |
- |
Bacillus cereus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.9 | L-2-aminobutanoate + H2O + NAD+ | - |
Bacillus cereus | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.9 | L-Ile + H2O + NAD+ | - |
Bacillus cereus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-Leu + H2O + NAD+ | - |
Bacillus cereus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-Met + H2O + NAD+ | - |
Bacillus cereus | 4-methylthio-2-oxobutyrate + NH3 + NADH + H+ | - |
r | |
1.4.1.9 | L-norleucine + H2O + NAD+ | - |
Bacillus cereus | 2-oxohexanoate + NH3 + NADH | - |
r | |
1.4.1.9 | L-norvaline + H2O + NAD+ | - |
Bacillus cereus | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.9 | L-Val + H2O + NAD+ | - |
Bacillus cereus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.9 | octamer | 8 * 39000, SDS-PAGE | Bacillus cereus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 8.5 | - |
reductive amination of 2-oxopentanoate and 3-methyl-2-oxobutanoate | Bacillus cereus |
1.4.1.9 | 9 | 9.5 | reductive amination of 4-methyl-2-oxopentanoate, 2-oxopentanoate and 2-oxomethionine | Bacillus cereus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 5.6 | 9.8 | 25°C, 24 h, maximal loss of 12% of the activity | Bacillus cereus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | additional information | no activity with NADPH | Bacillus cereus | |
1.4.1.9 | additional information | no activity with NADP+ | Bacillus cereus | |
1.4.1.9 | NAD+ | - |
Bacillus cereus | |
1.4.1.9 | NADH | - |
Bacillus cereus |