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Literature summary extracted from

  • Zink, M.W.; Sanwal, B.D.
    L-Leucine dehydrogenase (Bacillus cereus) (1970), Methods Enzymol., 17A, 799-802.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 PCMB
-
Bacillus cereus
1.4.1.9 Sulfide
-
Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.12
-
NADH
-
Bacillus cereus
1.4.1.9 0.16
-
NAD+
-
Bacillus cereus
1.4.1.9 2
-
L-Val
-
Bacillus cereus
1.4.1.9 2.2
-
3-methyl-2-oxobutanoate
-
Bacillus cereus
1.4.1.9 5.2
-
L-Ile
-
Bacillus cereus
1.4.1.9 6.2
-
L-Leu
-
Bacillus cereus
1.4.1.9 13
-
NH4+
-
Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 additional information
-
-
Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
1.4.1.9 -20°C, 50% glycerol, stable for 1 year Bacillus cereus
1.4.1.9 -20°C, stable for over 1 month Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-2-aminobutanoate + H2O + NAD+
-
Bacillus cereus 2-oxobutanoate + NH3 + NADH + H+
-
?
1.4.1.9 L-Ile + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Bacillus cereus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Val + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 11.3
-
oxidative deamination Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NAD+
-
Bacillus cereus
1.4.1.9 NADH
-
Bacillus cereus