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Literature summary extracted from

  • Brunhuber, N.M.W.; Thoden, J.B.; Blanchard, J.S.; Vanhooke, J.L.
    Rhodococcus L-Phenylalanine Dehydrogenase: Kinetics, Mechanism, and Structural Basis for Catalytic Specificity (2000), Biochemistry, 39, 9174-9187.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.20 D-Phe
-
 Rhodococcus sp.
1.4.1.20 L-Phe
-
 Rhodococcus sp.
1.4.1.20 L-Phenyllactate
-
 Rhodococcus sp.
1.4.1.20 NADH
-
 Rhodococcus sp.
1.4.1.20 NH4+
-
 Rhodococcus sp.
1.4.1.20 Phenylethylamine
-
 Rhodococcus sp.
1.4.1.20 phenylpropionate
-
 Rhodococcus sp.
1.4.1.20 phenylpyruvate
-
 Rhodococcus sp.
1.4.1.20 trans-cinnamate
-
 Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.20 40
-
 oxidized beta-nicotinamide hypoxanthine dinucleotide
-
 Rhodococcus sp.
1.4.1.20 50
-
 3-pyridinealdehyde-NAD+
-
 Rhodococcus sp.
1.4.1.20 60
-
 thioNAD+
-
 Rhodococcus sp.
1.4.1.20 90
-
 NAD+
-
 Rhodococcus sp.
1.4.1.20 110
-
 3-acetylpyridine-NAD+
-
 Rhodococcus sp.
1.4.1.20 170
-
 oxidized beta-nicotinamide guanine dinucleotide
-
 Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 39500
-
 4 * 39500, SDS-PAGE Rhodococcus sp.
1.4.1.20 150000
-
 gel filtration Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Rhodococcus sp.
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.20 L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH + H+ kinetic mechanism is ordered with NAD+ binding prior to Phe and the products being released in the order of ammonia, phenylpyruvate, and NADH Rhodococcus sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 L-Phe + H2O + NAD+
-
 Rhodococcus sp. phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-phenylalanine + H2O + 3-acetylpyridine-NAD+ 350% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + acetylpyridine-NADH
-
 ?
1.4.1.20 L-phenylalanine + H2O + 3-pyridinealdehyde-NAD+ 55% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + 3-pyridinealdehyde-NADH
-
 ?
1.4.1.20 L-phenylalanine + H2O + oxidized beta-nicotinamide guanine dinucleotide at 86% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + reduced beta-nicotinamide guanine dinucleotide
-
 ?
1.4.1.20 L-phenylalanine + H2O + oxidized beta-nicotinamide hypoxanthine dinucleotide at 86% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + reduced beta-nicotinamide hypoxanthine dinucleotide
-
 ?
1.4.1.20 L-phenylalanine + H2O + thio-NAD+ at 86% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + thio-NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.20 tetramer 4 * 39500, SDS-PAGE Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 additional information no activity with NADH Rhodococcus sp.
1.4.1.20 NAD+
-
 Rhodococcus sp.
1.4.1.20 NADH
-
 Rhodococcus sp.