BRENDA - Enzyme Database

Purification and characterization of a dimeric phenylalanine dehydrogenase from Rhodococcus maris K-18

Misono, H.; Yonezawa, J.; Nagata, S.; Nagasaki, S.; J. Bacteriol. 171, 30-36 (1989) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.4.1.20
20% glycerol, 0.25 M sodium malonate or 0.5 M sodium glutarate stabilize the enzyme during storage at 4°C
Dietzia maris
1.4.1.20
unstable in absence of glycerol and salt
Dietzia maris
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.20
3-Phenylpropionate
competitive with L-Phe
Dietzia maris
1.4.1.20
4-hydroxyphenylethylamine
competitive with L-Phe
Dietzia maris
1.4.1.20
alpha-methyl-DL-phenylalanine
-
Dietzia maris
1.4.1.20
Cu2+
slight
Dietzia maris
1.4.1.20
D-ethionine
-
Dietzia maris
1.4.1.20
D-Phe
competitive with L-Phe
Dietzia maris
1.4.1.20
D-Tyr
competitive with L-Phe
Dietzia maris
1.4.1.20
HgCl2
0.01 mM, complete inhibition
Dietzia maris
1.4.1.20
L-Phe
product inhibition
Dietzia maris
1.4.1.20
L-phenylglycine
competitive with L-Phe
Dietzia maris
1.4.1.20
NAD+
product inhibition
Dietzia maris
1.4.1.20
PCMB
0.001 mM, complete inhibition
Dietzia maris
1.4.1.20
phenylenediamine
-
Dietzia maris
1.4.1.20
Phenylethylamine
competitive with L-Phe
Dietzia maris
1.4.1.20
trans-cinnamate
competitive with L-Phe
Dietzia maris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.1.20
0.18
-
deamino-NAD+
-
Dietzia maris
1.4.1.20
0.18
-
thionicotinamide-NAD+
-
Dietzia maris
1.4.1.20
0.25
-
NAD+
-
Dietzia maris
1.4.1.20
0.25
-
3-acetylpyridine-NAD+
-
Dietzia maris
1.4.1.20
0.5
-
phenylpyruvate
-
Dietzia maris
1.4.1.20
1.3
-
4-hydroxyphenylpyruvate
-
Dietzia maris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.1.20
36000
-
2 * 36000, SDS-PAGE
Dietzia maris
1.4.1.20
70000
-
gel filtration
Dietzia maris
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.4.1.20
Dietzia maris
-
K-18
-
1.4.1.20
Dietzia maris K-18
-
K-18
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.4.1.20
-
Dietzia maris
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
1.4.1.20
L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH + H+
the reductive amination proceeds through a sequential ordered ternary-binary mechanism
Dietzia maris
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.1.20
65.2
-
-
Dietzia maris
Storage Stability
EC Number
Storage Stability
Organism
1.4.1.20
4°C, 0.25 M sodium malonate, stable for 1 month
Dietzia maris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.4.1.20
4-hydroxyphenylpyruvate + NH3 + NADH + H+
-
349636
Dietzia maris
L-tyrosine + NAD+ + H2O
-
-
-
r
1.4.1.20
L-alpha-amino-beta-phenylbutyrate + H2O + NAD+
7.0% of the activity with L-Phe
349636
Dietzia maris
2-oxo-3-phenylbutyrate + NADH + NH3
-
-
-
?
1.4.1.20
L-ethionine + H2O + NAD+
13.0% of the activity with L-Phe
349636
Dietzia maris
4-ethylthio-2-oxobutanoate + NH3 + NADH
-
-
-
?
1.4.1.20
L-norleucine + H2O + NAD+
15.6% of the activity with L-Phe
349636
Dietzia maris
2-oxohexanoic acid + NADH + NH3
-
-
-
?
1.4.1.20
L-Phe + H2O + NAD+
the reductive amination proceeds through a sequential ordered ternary-binary mechanism
349636
Dietzia maris
phenylpyruvate + NH3 + NADH
-
-
-
r
1.4.1.20
L-Phe + H2O + NAD+
the reductive amination proceeds through a sequential ordered ternary-binary mechanism
349636
Dietzia maris K-18
phenylpyruvate + NH3 + NADH
-
-
-
r
1.4.1.20
L-phenylalanine + H2O + 3-acetylpyridine-NAD+
at 241% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + acetylpyridine-NADH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + 3-acetylpyridine-NAD+
at 241% of the activity with NAD+
349636
Dietzia maris K-18
phenylpyruvate + NH3 + acetylpyridine-NADH
-
349636
Dietzia maris K-18
?
1.4.1.20
L-phenylalanine + H2O + 3-pyridinealdehyde-NAD+
at 9.2% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + 3-pyridinealdehyde-NADH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + deamino-NAD+
at 86% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + deamino-NADH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + deamino-NAD+
at 86% of the activity with NAD+
349636
Dietzia maris K-18
phenylpyruvate + NH3 + deamino-NADH
-
349636
Dietzia maris K-18
?
1.4.1.20
L-phenylalanine + H2O + NADP+
at 6.6% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + NADPH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + NADP+
at 6.6% of the activity with NAD+
349636
Dietzia maris K-18
phenylpyruvate + NH3 + NADPH
-
349636
Dietzia maris K-18
?
1.4.1.20
L-phenylalanine + H2O + thionicotinamide-NAD+
at 101% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + thionicotinamide-NADH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + thionicotinamide-NAD+
at 101% of the activity with NAD+
349636
Dietzia maris K-18
phenylpyruvate + NH3 + thionicotinamide-NADH
-
349636
Dietzia maris K-18
?
1.4.1.20
L-Trp + H2O + NAD+
7.5% of the activity with L-Phe
349636
Dietzia maris
indole-3-pyruvate + NH3 + NADH
-
-
-
?
1.4.1.20
m-fluoro-DL-phenylalanine + H2O + NAD+
7.7% of the activity with L-Phe
349636
Dietzia maris
3-(3-fluorophenyl)-2-oxopropionate + NADH + NH3
-
-
-
?
1.4.1.20
p-fluoro-DL-phenylalanine + H2O + NAD+
8.3% of the activity with L-Phe
349636
Dietzia maris
(4-fluorophenyl)-2-oxopropionate + NADH + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.1.20
dimer
2 * 36000, SDS-PAGE
Dietzia maris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.1.20
9.8
-
reductive amination of phenylpyruvate
Dietzia maris
1.4.1.20
10.8
-
oxidative deamination of L-Phe
Dietzia maris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.20
3-acetylpyridine-NAD+
241% of the activity with NAD+ in the reaction with L-Phe
Dietzia maris
1.4.1.20
3-pyridinealdehyde-NAD+
9.2% of the activity with NAD+ in the reaction with L-Phe
Dietzia maris
1.4.1.20
deamino-NAD+
86% of the activity with NAD+ in the reaction with L-Phe
Dietzia maris
1.4.1.20
NAD+
-
Dietzia maris
1.4.1.20
NADH
-
Dietzia maris
1.4.1.20
thionicotinamide-NAD+
101% of the activity with NAD+ in the reaction with L-Phe
Dietzia maris
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.1.20
0.14
-
D-Phe
-
Dietzia maris
1.4.1.20
1.52
-
Phenylethylamine
-
Dietzia maris
1.4.1.20
1.58
-
D-Tyr
-
Dietzia maris
1.4.1.20
1.76
-
D-ethionine
-
Dietzia maris
1.4.1.20
2.6
-
3-Phenylpropionate
-
Dietzia maris
1.4.1.20
4.36
-
phenylenediamine
-
Dietzia maris
1.4.1.20
8.32
-
L-phenylglycine
-
Dietzia maris
1.4.1.20
12.7
-
trans-cinnamic acid
-
Dietzia maris
1.4.1.20
13.7
-
4-hydroxyphenylethylamine
-
Dietzia maris
1.4.1.20
20.8
-
alpha-methyl-DL-phenylalanine
-
Dietzia maris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.20
3-acetylpyridine-NAD+
241% of the activity with NAD+ in the reaction with L-Phe
Dietzia maris
1.4.1.20
3-pyridinealdehyde-NAD+
9.2% of the activity with NAD+ in the reaction with L-Phe
Dietzia maris
1.4.1.20
deamino-NAD+
86% of the activity with NAD+ in the reaction with L-Phe
Dietzia maris
1.4.1.20
NAD+
-
Dietzia maris
1.4.1.20
NADH
-
Dietzia maris
1.4.1.20
thionicotinamide-NAD+
101% of the activity with NAD+ in the reaction with L-Phe
Dietzia maris
General Stability (protein specific)
EC Number
General Stability
Organism
1.4.1.20
20% glycerol, 0.25 M sodium malonate or 0.5 M sodium glutarate stabilize the enzyme during storage at 4°C
Dietzia maris
1.4.1.20
unstable in absence of glycerol and salt
Dietzia maris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.20
3-Phenylpropionate
competitive with L-Phe
Dietzia maris
1.4.1.20
4-hydroxyphenylethylamine
competitive with L-Phe
Dietzia maris
1.4.1.20
alpha-methyl-DL-phenylalanine
-
Dietzia maris
1.4.1.20
Cu2+
slight
Dietzia maris
1.4.1.20
D-ethionine
-
Dietzia maris
1.4.1.20
D-Phe
competitive with L-Phe
Dietzia maris
1.4.1.20
D-Tyr
competitive with L-Phe
Dietzia maris
1.4.1.20
HgCl2
0.01 mM, complete inhibition
Dietzia maris
1.4.1.20
L-Phe
product inhibition
Dietzia maris
1.4.1.20
L-phenylglycine
competitive with L-Phe
Dietzia maris
1.4.1.20
NAD+
product inhibition
Dietzia maris
1.4.1.20
PCMB
0.001 mM, complete inhibition
Dietzia maris
1.4.1.20
phenylenediamine
-
Dietzia maris
1.4.1.20
Phenylethylamine
competitive with L-Phe
Dietzia maris
1.4.1.20
trans-cinnamate
competitive with L-Phe
Dietzia maris
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.1.20
0.14
-
D-Phe
-
Dietzia maris
1.4.1.20
1.52
-
Phenylethylamine
-
Dietzia maris
1.4.1.20
1.58
-
D-Tyr
-
Dietzia maris
1.4.1.20
1.76
-
D-ethionine
-
Dietzia maris
1.4.1.20
2.6
-
3-Phenylpropionate
-
Dietzia maris
1.4.1.20
4.36
-
phenylenediamine
-
Dietzia maris
1.4.1.20
8.32
-
L-phenylglycine
-
Dietzia maris
1.4.1.20
12.7
-
trans-cinnamic acid
-
Dietzia maris
1.4.1.20
13.7
-
4-hydroxyphenylethylamine
-
Dietzia maris
1.4.1.20
20.8
-
alpha-methyl-DL-phenylalanine
-
Dietzia maris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.1.20
0.18
-
deamino-NAD+
-
Dietzia maris
1.4.1.20
0.18
-
thionicotinamide-NAD+
-
Dietzia maris
1.4.1.20
0.25
-
NAD+
-
Dietzia maris
1.4.1.20
0.25
-
3-acetylpyridine-NAD+
-
Dietzia maris
1.4.1.20
0.5
-
phenylpyruvate
-
Dietzia maris
1.4.1.20
1.3
-
4-hydroxyphenylpyruvate
-
Dietzia maris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.1.20
36000
-
2 * 36000, SDS-PAGE
Dietzia maris
1.4.1.20
70000
-
gel filtration
Dietzia maris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.1.20
-
Dietzia maris
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.1.20
65.2
-
-
Dietzia maris
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.4.1.20
4°C, 0.25 M sodium malonate, stable for 1 month
Dietzia maris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.4.1.20
4-hydroxyphenylpyruvate + NH3 + NADH + H+
-
349636
Dietzia maris
L-tyrosine + NAD+ + H2O
-
-
-
r
1.4.1.20
L-alpha-amino-beta-phenylbutyrate + H2O + NAD+
7.0% of the activity with L-Phe
349636
Dietzia maris
2-oxo-3-phenylbutyrate + NADH + NH3
-
-
-
?
1.4.1.20
L-ethionine + H2O + NAD+
13.0% of the activity with L-Phe
349636
Dietzia maris
4-ethylthio-2-oxobutanoate + NH3 + NADH
-
-
-
?
1.4.1.20
L-norleucine + H2O + NAD+
15.6% of the activity with L-Phe
349636
Dietzia maris
2-oxohexanoic acid + NADH + NH3
-
-
-
?
1.4.1.20
L-Phe + H2O + NAD+
the reductive amination proceeds through a sequential ordered ternary-binary mechanism
349636
Dietzia maris
phenylpyruvate + NH3 + NADH
-
-
-
r
1.4.1.20
L-Phe + H2O + NAD+
the reductive amination proceeds through a sequential ordered ternary-binary mechanism
349636
Dietzia maris K-18
phenylpyruvate + NH3 + NADH
-
-
-
r
1.4.1.20
L-phenylalanine + H2O + 3-acetylpyridine-NAD+
at 241% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + acetylpyridine-NADH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + 3-acetylpyridine-NAD+
at 241% of the activity with NAD+
349636
Dietzia maris K-18
phenylpyruvate + NH3 + acetylpyridine-NADH
-
349636
Dietzia maris K-18
?
1.4.1.20
L-phenylalanine + H2O + 3-pyridinealdehyde-NAD+
at 9.2% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + 3-pyridinealdehyde-NADH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + deamino-NAD+
at 86% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + deamino-NADH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + deamino-NAD+
at 86% of the activity with NAD+
349636
Dietzia maris K-18
phenylpyruvate + NH3 + deamino-NADH
-
349636
Dietzia maris K-18
?
1.4.1.20
L-phenylalanine + H2O + NADP+
at 6.6% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + NADPH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + NADP+
at 6.6% of the activity with NAD+
349636
Dietzia maris K-18
phenylpyruvate + NH3 + NADPH
-
349636
Dietzia maris K-18
?
1.4.1.20
L-phenylalanine + H2O + thionicotinamide-NAD+
at 101% of the activity with NAD+
349636
Dietzia maris
phenylpyruvate + NH3 + thionicotinamide-NADH
-
349636
Dietzia maris
?
1.4.1.20
L-phenylalanine + H2O + thionicotinamide-NAD+
at 101% of the activity with NAD+
349636
Dietzia maris K-18
phenylpyruvate + NH3 + thionicotinamide-NADH
-
349636
Dietzia maris K-18
?
1.4.1.20
L-Trp + H2O + NAD+
7.5% of the activity with L-Phe
349636
Dietzia maris
indole-3-pyruvate + NH3 + NADH
-
-
-
?
1.4.1.20
m-fluoro-DL-phenylalanine + H2O + NAD+
7.7% of the activity with L-Phe
349636
Dietzia maris
3-(3-fluorophenyl)-2-oxopropionate + NADH + NH3
-
-
-
?
1.4.1.20
p-fluoro-DL-phenylalanine + H2O + NAD+
8.3% of the activity with L-Phe
349636
Dietzia maris
(4-fluorophenyl)-2-oxopropionate + NADH + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.1.20
dimer
2 * 36000, SDS-PAGE
Dietzia maris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.1.20
9.8
-
reductive amination of phenylpyruvate
Dietzia maris
1.4.1.20
10.8
-
oxidative deamination of L-Phe
Dietzia maris