Any feedback?
Literature summary extracted from
- Purification and characterisation of a water-soluble ferrochelatase from Bacillus subtilis (1994), Eur. J. Biochem., 220, 201-208.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.98.1.1 | expressed in Escherichia coli | Bacillus subtilis |
| 4.99.1.9 | gene cloned in pUC18, expression in Escherichia coli JM109/pLUGlSe2 | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.99.1.9 | N-ethylmaleimide | 2 mM, 99% inactivation after incubation for 2 h. Protoporphyrin IX or Zn2+ at concentrations of 0.8 mM and 20 mM, respectively, does not protect | Bacillus subtilis |  |
| 4.99.1.9 | p-chloromercuriphenylsulfonate | - |
Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.98.1.1 | 0.008 | - |
protoporphyrin | - |
Bacillus subtilis | |
| 4.98.1.1 | 0.017 | - |
Zn2+ | - |
Bacillus subtilis | |
| 4.98.1.1 | 0.17 | - |
Cu2+ | - |
Bacillus subtilis | |
| 4.99.1.9 | 0.008 | - |
protoporphyrin IX | pH 7.2, 30°C. The Km-value for protoporphyrin IX is the same independent of whether Zn2+ or Fe2+ is used in the reaction | Bacillus subtilis | |
| 4.99.1.9 | 0.017 | - |
Zn2+ | pH 7.2, 30°C | Bacillus subtilis | |
| 4.99.1.9 | 0.17 | - |
Cu2+ | pH 7.2, 30°C | Bacillus subtilis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.98.1.1 | soluble | - |
Bacillus subtilis | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.98.1.1 | Cu2+ | - |
Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.98.1.1 | 35000 | - |
SDS-PAGE, gel filtration | Bacillus subtilis |
| 4.99.1.9 | 35348 | - |
1 * 35348, calculated from sequence | Bacillus subtilis |
| 4.99.1.9 | 40000 | - |
gel filtration | Bacillus subtilis |
| 4.99.1.9 | 40500 | - |
1 * 40500, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.98.1.1 | protoporphyrin IX + Fe2+ | Bacillus subtilis | final step of heme biosynthesis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.98.1.1 | Bacillus subtilis | - |
- |
- |
| 4.99.1.9 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.98.1.1 | expressed in Escherichia coli | Bacillus subtilis |
| 4.99.1.9 | - |
Bacillus subtilis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.99.1.9 | -20°C, the purified enzyme is stable for at least five months | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.98.1.1 | protoporphyrin + Cu2+ | - |
Bacillus subtilis | Cu2+-protoporphyrin + H+ | - |
? | |
| 4.98.1.1 | protoporphyrin + Fe2+ | - |
Bacillus subtilis | protoheme + 2 H+ | - |
? | |
| 4.98.1.1 | protoporphyrin + Zn2+ | - |
Bacillus subtilis | Zn2+-protoporphyrin + H+ | - |
? | |
| 4.98.1.1 | protoporphyrin IX + Fe2+ | final step of heme biosynthesis | Bacillus subtilis | ? | - |
? | |
| 4.99.1.9 | protoporphyrin IX + Cu2+ | - |
Bacillus subtilis | Cu-protoporphyrin IX + 2 H+ | - |
? | |
| 4.99.1.9 | protoporphyrin IX + Fe2+ | the enzyme shows no detectable activity with Ca2+, Co2+, Fe3+, Mg2+, or Rb+ | Bacillus subtilis | Fe-protoporphyrin IX + 2 H+ | - |
? | |
| 4.99.1.9 | protoporphyrin IX + Zn2+ | - |
Bacillus subtilis | Zn-protoporphyrin IX + 2 H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.98.1.1 | monomer | SDS-PAGE, gel filtration | Bacillus subtilis |
| 4.99.1.9 | monomer | 1 * 40500, SDS-PAGE | Bacillus subtilis |
| 4.99.1.9 | monomer | 1 * 35348, calculated from sequence | Bacillus subtilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.99.1.9 | hemH | - |
Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.99.1.9 | 30 | - |
assay at | Bacillus subtilis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.98.1.1 | 0.3 | 0.483 | protoporphyrin | - |
Bacillus subtilis | |
| 4.99.1.9 | 0.4 | - |
protoporphyrin IX | pH 7.2, 30°C. The Km-value for protoporphyrin IX is the same independent of whether Zn2+ or Fe2+ is used in the reaction | Bacillus subtilis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.98.1.1 | 7.2 | - |
- |
Bacillus subtilis |
| 4.99.1.9 | 7.2 | - |
assay at | Bacillus subtilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.99.1.9 | 6.5 | 8 | pH 6.5: about 50% of maximal activity, pH 8.0: about 50% of maximal activity | Bacillus subtilis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.99.1.9 | metabolism | the enzyme participates in heme biosynthesis | Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
