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Literature summary extracted from

  • Rowell, P.; Stewart, W.D.P.
    Alanine dehydrogenase of the N2-fixing blue-green alga, Anbabaena cylindrica (1976), Arch. Microbiol., 107, 115-124.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 Borate
-
 Anabaena cylindrica
1.4.1.1 glycine
-
 Anabaena cylindrica
1.4.1.1 L-Ala
-
 Anabaena cylindrica
1.4.1.1 L-Cys
-
 Anabaena cylindrica
1.4.1.1 L-Ser
-
 Anabaena cylindrica
1.4.1.1 PCMB
-
 Anabaena cylindrica
1.4.1.1 Zn2+
-
 Anabaena cylindrica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.11
-
 pyruvate
-
 Anabaena cylindrica
1.4.1.1 0.4
-
 L-Ala
-
 Anabaena cylindrica
1.4.1.1 3
-
 oxaloacetate
-
 Anabaena cylindrica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 43000
-
 6 * 43000, SDS-PAGE Anabaena cylindrica
1.4.1.1 270000
-
 gel filtration Anabaena cylindrica

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Anabaena cylindrica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Anabaena cylindrica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.1 heterocyst
-
 Anabaena cylindrica
-
1.4.1.1 vegetative
-
 Anabaena cylindrica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 38
-
-
 Anabaena cylindrica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Anabaena cylindrica pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-norvaline + H2O + NAD+
-
 Anabaena cylindrica 2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.1 oxaloacetate + NH3 + NADH
-
 Anabaena cylindrica L-Asp + H2O + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 hexamer 6 * 43000, SDS-PAGE Anabaena cylindrica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8
-
 reductive amination Anabaena cylindrica
1.4.1.1 9.6
-
 oxidative deamination Anabaena cylindrica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.1 6.8 8.8 pH 6.8: about 50% of maximal activity, pH 8.8: about 60% of maximal activity, reductive amination Anabaena cylindrica
1.4.1.1 8.5 10.6 pH 8.5: about 60% of maximal activity, pH 10.6: about 40% of maximal activity, oxidative deamination Anabaena cylindrica

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Anabaena cylindrica
1.4.1.1 NADH
-
 Anabaena cylindrica