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Literature summary extracted from

  • Keradjopoulos, D.; Holldorf, A.W.
    Purification and properties of alanine dehydrogenase from Halobacterium salinarium (1979), Biochim. Biophys. Acta, 570, 1-10.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 F-
-
 Halobacterium salinarum
1.4.1.1 I-
-
 Halobacterium salinarum
1.4.1.1 perchlorate
-
 Halobacterium salinarum
1.4.1.1 thiocyanate
-
 Halobacterium salinarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.1 CdCl2 stimulates Halobacterium salinarum
1.4.1.1 Cs+ CsCl, weak stimulation Halobacterium salinarum
1.4.1.1 KCl KCl stimulates Halobacterium salinarum
1.4.1.1 Li+ LiCl, weak stimulation Halobacterium salinarum
1.4.1.1 additional information highest activation by Cl-, much smaller effect occurs in presence of Br-, nitrate, or sulfate Halobacterium salinarum
1.4.1.1 NaCl NaCl, active only in presence of high concentrations Halobacterium salinarum
1.4.1.1 RbCl stimulates Halobacterium salinarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 58000
-
 non-denaturing PAGE Halobacterium salinarum
1.4.1.1 60000
-
 gel filtration Halobacterium salinarum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Halobacterium salinarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Halobacterium salinarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 178
-
-
 Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 2-oxobutanoate + NH3 + NADH
-
 Halobacterium salinarum 2-aminobutanoate + H2O + NAD+
-
 r
1.4.1.1 glyoxylate + NH3 + NADH
-
 Halobacterium salinarum aminoacetate + NAD+ + H2O
-
 ?
1.4.1.1 hydroxypyruvate + NH3 + NADH
-
 Halobacterium salinarum L-Ser + NAD+ + H2O
-
 ?
1.4.1.1 L-2-aminobutanoate + H2O + NAD+
-
 Halobacterium salinarum 2-oxobutanoate + NH3 + NADH + H+
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Halobacterium salinarum pyruvate + NH3 + NADH
-
 r
1.4.1.1 oxaloacetate + NH3 + NADH
-
 Halobacterium salinarum L-Asp + H2O + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 monomer 1 * 58000-60000, SDS-PAGE Halobacterium salinarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 45
-
 presence of 3.5 M NaNO3 Halobacterium salinarum
1.4.1.1 65
-
 in presence of 3.5 M NaCl Halobacterium salinarum
1.4.1.1 70
-
 3.5 M KCl Halobacterium salinarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 additional information
-
 thermal stability is considerably lower in presence of KCl than in presence of NaCl Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 9
-
 both directions Halobacterium salinarum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Halobacterium salinarum
1.4.1.1 NADH
-
 Halobacterium salinarum