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Literature summary extracted from

  • Vali, Z.; Kilar, F.; Lakatos, S.; Venyaminov, S.A.; Zavodszky, P.
    L-Alanine dehydrogenase from Thermus thermophilus (1980), Biochim. Biophys. Acta, 615, 34-47.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.1
-
 Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 D-Ala
-
 Bacillus subtilis
1.4.1.1 D-Ala
-
 Thermus thermophilus
1.4.1.1 D-Cys
-
 Bacillus subtilis
1.4.1.1 D-Cys
-
 Thermus thermophilus
1.4.1.1 L-Cys
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.023
-
 NADH
-
 Bacillus subtilis
1.4.1.1 0.035
-
 NADH
-
 Bacillus subtilis
1.4.1.1 0.035
-
 NADH
-
 Thermus thermophilus
1.4.1.1 0.12
-
 NAD+
-
 Thermus thermophilus
1.4.1.1 0.18
-
 NAD+
-
 Bacillus subtilis
1.4.1.1 0.54
-
 pyruvate
-
 Bacillus subtilis
1.4.1.1 0.75
-
 pyruvate
-
 Thermus thermophilus
1.4.1.1 1.7
-
 L-Ala
-
 Bacillus subtilis
1.4.1.1 4.2
-
 L-Ala
-
 Thermus thermophilus
1.4.1.1 38
-
 NH4+
-
 Bacillus subtilis
1.4.1.1 59
-
 NH3
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 48000
-
 6 * 48000, SDS-PAGE Thermus thermophilus
1.4.1.1 290000
-
 equilibrium sedimentation Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Bacillus subtilis
-
-
-
1.4.1.1 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Bacillus subtilis pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Thermus thermophilus pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ile + H2O + NAD+ 0.1% of the activity with L-Ala Thermus thermophilus 2-oxo-3-methylpentanoate + NH3 + NADH
-
 ?
1.4.1.1 L-Ile + H2O + NAD+ 5% of the activity with L-Ala Bacillus subtilis 2-oxo-3-methylpentanoate + NH3 + NADH
-
 ?
1.4.1.1 L-Ser + H2O + NAD+
-
 Bacillus subtilis 3-hydroxypyruvate + NH3 + NADH
-
 ?
1.4.1.1 L-Ser + H2O + NAD+
-
 Thermus thermophilus 3-hydroxypyruvate + NH3 + NADH
-
 ?
1.4.1.1 L-Val + H2O + NAD+ 9% of the activity with L-Ala Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.1 L-Val + H2O + NAD+ 0.2% of the activity with L-Ala Thermus thermophilus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 hexamer
-
 Bacillus subtilis
1.4.1.1 hexamer 6 * 48000, SDS-PAGE Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 63
-
 melting temperature Bacillus subtilis
1.4.1.1 86
-
 melting temperature Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8
-
-
 Thermus thermophilus
1.4.1.1 8.5
-
-
 Thermus thermophilus
1.4.1.1 8.5
-
 reductive amination of pyruvate Bacillus subtilis
1.4.1.1 10.5
-
 deamination of L-Ala Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Bacillus subtilis
1.4.1.1 NAD+
-
 Thermus thermophilus
1.4.1.1 NADH
-
 Bacillus subtilis
1.4.1.1 NADH
-
 Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.1 0.64
-
 D-Cys
-
 Bacillus subtilis
1.4.1.1 0.9
-
 D-Cys
-
 Bacillus subtilis
1.4.1.1 5
-
 D-Ala
-
 Bacillus subtilis
1.4.1.1 20
-
 D-Ala
-
 Bacillus subtilis