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Literature summary extracted from

  • Sawa, Y.; Tani, M.; Murata, K.; Shibata, H.; Ochiai, H.
    Purification and characterization of alanine dehydrogenase from a cyanobacterium, Phormidium lapideum (1994), J. Biochem., 116, 995-1000.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.1 2-mercaptoethanol activation Phormidium lapideum

General Stability

EC Number General Stability Organism
1.4.1.1 partial inactivation by dilution Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 Cd2+
-
 Phormidium lapideum
1.4.1.1 Cibacron blue
-
 Phormidium lapideum
1.4.1.1 Cu2+
-
 Phormidium lapideum
1.4.1.1 Hg2+
-
 Phormidium lapideum
1.4.1.1 L-Ala
-
 Phormidium lapideum
1.4.1.1 L-Phe reductive amination of pyruvate, 10 mM, 28% inhibition Phormidium lapideum
1.4.1.1 L-Ser reductive amination of pyruvate Phormidium lapideum
1.4.1.1 L-Thr reductive amination of pyruvate, 10 mM, 37% inhibition Phormidium lapideum
1.4.1.1 L-Trp reductive amination of pyruvate, 10 mM, 29% inhibition Phormidium lapideum
1.4.1.1 PCMB
-
 Bacillus subtilis
1.4.1.1 PCMB
-
 Phormidium lapideum
1.4.1.1 pyridoxal 5'-phosphate
-
 Phormidium lapideum
1.4.1.1 Zn2+
-
 Bacillus subtilis
1.4.1.1 Zn2+
-
 Phormidium lapideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.02
-
 NADH
-
 Phormidium lapideum
1.4.1.1 0.04
-
 NAD+
-
 Phormidium lapideum
1.4.1.1 0.33
-
 pyruvate
-
 Phormidium lapideum
1.4.1.1 5
-
 L-Ala
-
 Phormidium lapideum
1.4.1.1 111
-
 NH4+
-
 Phormidium lapideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 41000
-
 6 * 41000, SDS-PAGE Phormidium lapideum
1.4.1.1 240000
-
 gel filtration Phormidium lapideum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Bacillus subtilis
-
-
-
1.4.1.1 Phormidium lapideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Phormidium lapideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 183
-
-
 Phormidium lapideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 2-oxobutanoate + NH3 + NADH 4.1% of the activity with pyruvate Phormidium lapideum 2-aminobutanoate + H2O + NAD+
-
 ?
1.4.1.1 3-hydroxypyruvate + NH3 + NADH 15.4% of the activity with pyruvate Phormidium lapideum Ser + H2O + NAD+
-
 ?
1.4.1.1 glyoxylate + NH3 + NADH 1.2% of the activity with pyruvate Phormidium lapideum aminoacetate + NAD+ + H2O
-
 ?
1.4.1.1 L-Ala + H2O + NAD+
-
 Bacillus subtilis pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Phormidium lapideum pyruvate + NH3 + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.1 hexamer
-
 Bacillus subtilis
1.4.1.1 hexamer 6 * 41000, SDS-PAGE Phormidium lapideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 60 70 reductive amination Phormidium lapideum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 50
-
 5 min, pH 6.0-9.0, stable Phormidium lapideum
1.4.1.1 60
-
 10 min, in presence of 0.3 mM NADH, 30% loss of activity Phormidium lapideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8.4
-
 reductive amination Phormidium lapideum
1.4.1.1 9.2
-
 oxidative deamination Phormidium lapideum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.1 7.2 9.2 pH 7.2: about 40% of maximal activity, pH 9.2: 40% of maximal activity, reductive amination of pyruvate Phormidium lapideum
1.4.1.1 7.8 10 pH 7.8: about 50% of maximal activity, pH 10.0: about 65% of maximal activity, oxidative deamination of L-Ala Phormidium lapideum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.1 6 9 50°C, 5 min, stable Phormidium lapideum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Bacillus subtilis
1.4.1.1 NAD+
-
 Phormidium lapideum
1.4.1.1 NADH
-
 Bacillus subtilis
1.4.1.1 NADH
-
 Phormidium lapideum