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Literature summary extracted from

  • Ohshima, T.; Wandrey, C.; Sugiura, M.; Soda, K.
    Screening of thermostable leucine and alanine dehydrogenases in thermophilic Bacillus strains (1985), Biotechnol. Lett., 7, 871-876.
No PubMed abstract available

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Bacillus sp. (in: Bacteria)
-
 screening of thermostable alanine dehydrogenase in thermophilic Bacillus strains
-
1.4.1.1 Geobacillus stearothermophilus
-
-
-
1.4.1.1 Lysinibacillus sphaericus
-
-
-
1.4.1.1 Weizmannia coagulans
-
-
-
1.4.1.9 Bacillus sp. (in: Bacteria)
-
 DSM 405, DSM 730 and DSM 1521
-
1.4.1.9 Lysinibacillus sphaericus
-
 DSM 462
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Geobacillus stearothermophilus pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Lysinibacillus sphaericus pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Weizmannia coagulans pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Bacillus sp. (in: Bacteria) pyruvate + NH3 + NADH
-
 r
1.4.1.9 L-Leu + H2O + NAD+
-
 Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.9 L-Leu + H2O + NAD+
-
 Bacillus sp. (in: Bacteria) 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 additional information
-
 screening of thermostable alanine dehydrogenase in thermophilic Bacillus strains Bacillus sp. (in: Bacteria)
1.4.1.1 75
-
 5 min, stable up to Geobacillus stearothermophilus
1.4.1.1 75
-
 5 min, stable up to Lysinibacillus sphaericus
1.4.1.1 75
-
 5 min, stable up to Weizmannia coagulans
1.4.1.1 75
-
 5 min, stable up to Bacillus sp. (in: Bacteria)
1.4.1.1 83
-
 5 min, 50% loss of activity Geobacillus stearothermophilus
1.4.1.1 83
-
 5 min, 50% loss of activity Lysinibacillus sphaericus
1.4.1.1 83
-
 5 min, 50% loss of activity Weizmannia coagulans
1.4.1.1 83
-
 5 min, 50% loss of activity Bacillus sp. (in: Bacteria)
1.4.1.9 75
-
 5 min, stable Lysinibacillus sphaericus
1.4.1.9 75
-
 5 min, stable Bacillus sp. (in: Bacteria)
1.4.1.9 83
-
 5 min, 50% loss of activity Lysinibacillus sphaericus
1.4.1.9 83
-
 5 min, 50% loss of activity Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Geobacillus stearothermophilus
1.4.1.1 NAD+
-
 Lysinibacillus sphaericus
1.4.1.1 NAD+
-
 Weizmannia coagulans
1.4.1.1 NAD+
-
 Bacillus sp. (in: Bacteria)
1.4.1.1 NADH
-
 Geobacillus stearothermophilus
1.4.1.1 NADH
-
 Lysinibacillus sphaericus
1.4.1.1 NADH
-
 Weizmannia coagulans
1.4.1.1 NADH
-
 Bacillus sp. (in: Bacteria)
1.4.1.9 NAD+
-
 Lysinibacillus sphaericus
1.4.1.9 NAD+
-
 Bacillus sp. (in: Bacteria)
1.4.1.9 NADH
-
 Lysinibacillus sphaericus
1.4.1.9 NADH
-
 Bacillus sp. (in: Bacteria)