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Literature summary extracted from

  • Bellion, E.; Tan, F.
    An NAD+-dependent alanine dehydrogenase from a methylotrophic bacterium (1987), Biochem. J., 244, 565-570.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 3-Bromopyruvate
-
 Pseudomonas sp.
1.4.1.1 5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas sp.
1.4.1.1 HgCl2
-
 Pseudomonas sp.
1.4.1.1 Hydroxypyruvate
-
 Pseudomonas sp.
1.4.1.1 L-Ala
-
 Pseudomonas sp.
1.4.1.1 L-Ser
-
 Pseudomonas sp.
1.4.1.1 NAD+ 0.1 mM, 35% inhibition Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 53000
-
 4 * 53000, SDS-PAGE Pseudomonas sp.
1.4.1.1 217000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+ Pseudomonas sp. role in NH3 assimilation and alanine catabolism pyruvate + NH3 + NADH
-
 ?
1.4.1.1 L-Ala + H2O + NAD+ Pseudomonas sp. MA role in NH3 assimilation and alanine catabolism pyruvate + NH3 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Pseudomonas sp.
-
-
-
1.4.1.1 Pseudomonas sp. MA
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 129
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+ rate of the deaminating reaction is only 2.2% of the aminating reaction Pseudomonas sp. pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+ role in NH3 assimilation and alanine catabolism Pseudomonas sp. pyruvate + NH3 + NADH
-
 ?
1.4.1.1 L-Ala + H2O + NAD+ rate of the deaminating reaction is only 2.2% of the aminating reaction Pseudomonas sp. MA pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+ role in NH3 assimilation and alanine catabolism Pseudomonas sp. MA pyruvate + NH3 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 tetramer 4 * 53000, SDS-PAGE Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 9
-
 reductive amination Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Pseudomonas sp.
1.4.1.1 NADH
-
 Pseudomonas sp.