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Literature summary extracted from

  • Honorat, A.; Monot, F.; Ballerini, D.
    Synthesis of L-alanine and L-valine by enzyme systems from Bacillus megaterium (1990), Enzyme Microb. Technol., 12, 515-520.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 CoCl2
-
Priestia megaterium
1.4.1.1 CuSO4
-
Priestia megaterium
1.4.1.1 HgCl2
-
Priestia megaterium
1.4.1.1 pyruvate substrate inhibition Priestia megaterium
1.4.1.8 2-oxoisovalerate 65 mM, 50% loss of specific activity Priestia megaterium
1.4.1.8 CoCl2
-
Priestia megaterium
1.4.1.8 Cu2+ CuSO4: less inhibition compared with HgCl2 and CoCl2 Priestia megaterium
1.4.1.8 Hg2+
-
Priestia megaterium
1.4.1.8 L-valine 10 mM, about 50% loss of specific activity Priestia megaterium
1.4.1.8 Mn2+ MnCl2: less inhibition compared with HgCl2 and CoCl2 Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.09
-
NADH
-
Priestia megaterium
1.4.1.1 0.55
-
pyruvate
-
Priestia megaterium
1.4.1.1 235
-
NH4+
-
Priestia megaterium
1.4.1.8 0.05
-
NADH
-
Priestia megaterium
1.4.1.8 1.5
-
2-oxoisovalerate
-
Priestia megaterium
1.4.1.8 153
-
NH4+
-
Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Priestia megaterium
-
-
-
1.4.1.8 Priestia megaterium
-
strain ATCC 39118
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
Priestia megaterium
1.4.1.8
-
Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 additional information
-
-
Priestia megaterium
1.4.1.1 11.2
-
-
Priestia megaterium
1.4.1.8 7.8
-
-
Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 2-oxoglutarate + NH3 + NADH 11.7% of the activity with pyruvate Priestia megaterium L-Gln + NAD+ + H2O
-
?
1.4.1.1 3-bromopyruvate + NH3 + NADH 5.3% of the activity with pyruvate Priestia megaterium 2-amino-3-bromopropanoate + NAD+ + H2O
-
?
1.4.1.1 4-methyl-2-oxopentanoate + NH3 + NADH 1.6% of the activity with pyruvate Priestia megaterium 2-amino-4-methylpentanoate + NAD+ + H2O
-
?
1.4.1.1 D-Ala + H2O + NAD+ 32.3% of the activity with L-Ala Priestia megaterium pyruvate + NH3 + NADH
-
?
1.4.1.1 D-Ser + H2O + NAD+ 1.3% of the activity with L-Ser Priestia megaterium 3-hydroxypyruvate + NH3 + NADH
-
?
1.4.1.1 glyoxylate + NH3 + NADH 6.2% of the activity with pyruvate Priestia megaterium aminoacetate + NAD+ + H2O
-
?
1.4.1.1 L-Ala + H2O + NAD+
-
Priestia megaterium pyruvate + NH3 + NADH
-
r
1.4.1.1 L-Ser + H2O + NAD+ 7.9% of the activity with L-Ala Priestia megaterium 3-hydroxypyruvate + NH3 + NADH
-
?
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ 9% of the activity with L-leucine Priestia megaterium 2-oxobutyrate + NADH + NH3 reductive amination: 28.1% of the activity with 2-oxoisovalerate ?
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ 9% of the activity with L-leucine Priestia megaterium 2-oxobutyrate + NADH + NH3 reductive amination: 28.1% of the activity with 2-oxoisovalerate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ 9% of the activity with L-leucine Priestia megaterium 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate ?
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ 9% of the activity with L-leucine Priestia megaterium 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Priestia megaterium 2-oxobutyrate + NADH + NH3 reductive amination: 28.1% of the activity with 2-oxoisovalerate ?
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Priestia megaterium 2-oxobutyrate + NADH + NH3 reductive amination: 28.1% of the activity with 2-oxoisovalerate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Priestia megaterium 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate ?
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Priestia megaterium 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
1.4.1.8 L-alanine + H2O + NAD+ 3% of the activity with L-valine Priestia megaterium pyruvate + NH3 + NADH reductive amination: 2% of the activity with 2-oxoisovalerate r
1.4.1.8 L-alanine + H2O + NAD+ 3% of the activity with L-valine Priestia megaterium pyruvate + NH3 + NADH pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate r
1.4.1.8 L-isoleucine + H2O + NAD+ 61.3% of the activity with L-leucine Priestia megaterium 3-methyl-2-oxopentanoate + NADH + NH3 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate r
1.4.1.8 L-isoleucine + H2O + NAD+ 61.3% of the activity with L-leucine Priestia megaterium 3-methyl-2-oxopentanoate + NADH + NH3 reductive amination: 21.9% of the activity with 2-oxoisovalerate r
1.4.1.8 L-leucine + H2O + NAD+ preferred substrate Priestia megaterium 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
1.4.1.8 L-leucine + H2O + NAD+ preferred substrate Priestia megaterium 2-oxoisocaproate + NADH + NH3 reductive amination: 21.1% of the activity with 2-oxoisovalerate r
1.4.1.8 L-norleucine + H2O + NAD+ 4.7% of the activity with L-valine Priestia megaterium 2-oxocaproate + NADH + NH3 reductive amination: 9.7% of the activity with 2-oxoisovalerate r
1.4.1.8 L-norleucine + H2O + NAD+ 4.7% of the activity with L-valine Priestia megaterium 2-oxocaproate + NADH + NH3 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate r
1.4.1.8 L-norvaline + H2O + NAD+ 47.2% of the activity with L-leucine Priestia megaterium 2-oxovalerate + NH3 + NADH reductive amination: 37% of the activity with 2-oxoisovalerate r
1.4.1.8 L-norvaline + H2O + NAD+ 47.2% of the activity with L-leucine Priestia megaterium 2-oxovalerate + NH3 + NADH 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Priestia megaterium 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Priestia megaterium 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Priestia megaterium 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
1.4.1.8 L-valine + NAD(P)+ + H2O 88.9% of the activity with L-leucine Priestia megaterium 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O 88.9% of the activity with L-leucine Priestia megaterium 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O 88.9% of the activity with L-leucine Priestia megaterium 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
1.4.1.8 additional information no activity with the D-isomers of the substrates Priestia megaterium additional information
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 52
-
-
Priestia megaterium
1.4.1.8 47
-
-
Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 30
-
pH 7.5, half-life: 22 days Priestia megaterium
1.4.1.8 30
-
0.75 M ammonia buffer pH 8.5, half-life: 12 days Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8
-
reductive amination Priestia megaterium
1.4.1.1 10.5
-
deamination of L-Ala Priestia megaterium
1.4.1.8 8.5
-
reductive amination Priestia megaterium
1.4.1.8 10.5
-
oxidative deamination of L-valine Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
Priestia megaterium
1.4.1.1 NADH
-
Priestia megaterium
1.4.1.8 NAD+
-
Priestia megaterium
1.4.1.8 NADH NADH could not be replaced with NADPH for the reductive amination Priestia megaterium