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Literature summary extracted from

  • Lecain, E.; Chenivesse, X.; Spagnoli, R.; Pompon, D.
    Cloning by metabolic interference in yeast and enzymic characterization of Arabidopsis thaliana sterol DELTA7-reductase (1996), J. Biol. Chem., 271, 10866-10873.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.21 expression of cDNA in Saccharomyces cerevisiae and overexpression of recombinant protein Escherichia coli, sequence analysis Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.21 cytosol low activity Arabidopsis thaliana 5829
-
1.3.1.21 microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.21 cholesta-5,7-dien-3-beta-ol + NADPH Arabidopsis thaliana highly regiospecific for DELTA7-bond reduction, but wide substrate specificity cholesterol + NADP+
-
 ?
1.3.1.21 ergosta-5,7-diene-3beta-ol + NADPH Arabidopsis thaliana also ergosta-5,24(28)-, ergosta-5,22,24(28)-eneols and cholesta-5,24- or cholesta-5,22,24-eneols can be formed in vivo in the mutants of Saccharomyces cerevisiae ergosta-5-ene-3beta-ol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.21 Arabidopsis thaliana Q9LDU6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.21 cholesta-5,7-dien-3-beta-ol + NADPH highly regiospecific for DELTA7-bond reduction, but wide substrate specificity Arabidopsis thaliana cholesterol + NADP+
-
 ?
1.3.1.21 cholesta-5,7-dien-3beta-ol + NADPH wide substrate spectrum Arabidopsis thaliana cholesterol + NADP+
-
 ?
1.3.1.21 ergosta-5,7,22-trien-3beta-ol + NADPH i.e. ergosterol Arabidopsis thaliana ergosta-5,22-diene-3beta-ol + NADP+
-
 ?
1.3.1.21 ergosta-5,7-diene-3beta-ol + NADPH
-
 Arabidopsis thaliana ergosta-5-eneol + NADP+
-
 ?
1.3.1.21 ergosta-5,7-diene-3beta-ol + NADPH also ergosta-5,24(28)-, ergosta-5,22,24(28)-eneols and cholesta-5,24- or cholesta-5,22,24-eneols can be formed in vivo in the mutants of Saccharomyces cerevisiae Arabidopsis thaliana ergosta-5-ene-3beta-ol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.21 sterol DELTA7-reductase
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.21 NADPH no activity with NADH Arabidopsis thaliana