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Literature summary extracted from
- Expression and characterization of four recombinant human dihydrodiol dehydrogenase isoforms: oxidation of trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene to the activated o-quinone metabolite benzo[a]pyrene-7,8-dione (1998), Biochemistry, 37, 6781-6790.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.3.1.20 | DD1, DD2, DD4, type 2 3alpha-hydroxysteroid/dihydrodiol dehydrogenase, expression in Escherichia coli | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.20 | 0.0035 | - |
androsterone | DD4, DD2 | Homo sapiens |  |
| 1.3.1.20 | 0.017 | - |
trans-benzene dihydrodiol | DD4 | Homo sapiens | |
| 1.3.1.20 | 0.022 | - |
1-Acenaphthenol | DD1, DD2 | Homo sapiens | |
| 1.3.1.20 | 0.048 | - |
1-Indanol | DD1 | Homo sapiens | |
| 1.3.1.20 | 0.07 | - |
1-Acenaphthenol | 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | Homo sapiens | |
| 1.3.1.20 | 0.14 | - |
1-Acenaphthenol | DD4 | Homo sapiens | |
| 1.3.1.20 | 0.15 | - |
trans-benzene dihydrodiol | DD1 | Homo sapiens | |
| 1.3.1.20 | 0.17 | - |
1-Indanol | DD4 | Homo sapiens | |
| 1.3.1.20 | 0.32 | - |
trans-benzene dihydrodiol | 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | Homo sapiens | |
| 1.3.1.20 | 0.94 | - |
1-Indanol | DD2 | Homo sapiens | |
| 1.3.1.20 | 1.4 | - |
1-Indanol | 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | Homo sapiens | |
| 1.3.1.20 | 1.8 | - |
trans-benzene dihydrodiol | DD2 | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.20 | Homo sapiens | P42330 | DD1, DD2, DD4, type 2 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.20 | DD1, DD2, DD4 and 3alpha-hydroxysteroid dehydrogenase/dehydrodiol dehydrogenase, all recombinant from Escherichia coli | Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.3.1.20 | trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | isoforms belong to aldo-keto reductase superfamily | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.3.1.20 | liver | cDNAs isolated from HepG2 hepatoma cell line | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.20 | 0.21 | - |
DD4, substrate androsterone | Homo sapiens |
| 1.3.1.20 | 2.1 | - |
recombinant DD1, substrate 1-acenaphthenol | Homo sapiens |
| 1.3.1.20 | 2.5 | - |
recombinant DD2, substrate 1-acenaphthenol | Homo sapiens |
| 1.3.1.20 | 2.8 | - |
recombinant 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.20 | (S)-indan-1-ol + NADP+ | - |
Homo sapiens | ? | - |
? | |
| 1.3.1.20 | 1-acenaphthenol + NAD(P)+ | recombinant: DD1, DD2, and 3alpha-hydroysteroid dehydrogenase/dihydrodiol dehydrogenase | Homo sapiens | ? | - |
? | |
| 1.3.1.20 | 1-indanol + NADP+ | - |
Homo sapiens | 3H-inden-1-ol + NADPH | - |
? | |
| 1.3.1.20 | androsterone + NADPH | recombinant DD4 | Homo sapiens | ? | - |
? | |
| 1.3.1.20 | trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+ | - |
Homo sapiens | catechol + NAD(P)H | - |
? | |
| 1.3.1.20 | trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene + NADP+ | DD1, DD2, DD4, 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | Homo sapiens | benzo[a]-pyrene-7,8-dione + NADPH | product is potent carcinogen | ? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.20 | 0.00667 | - |
androsterone | DD2 | Homo sapiens | |
| 1.3.1.20 | 0.015 | - |
trans-benzene dihydrodiol | 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | Homo sapiens | |
| 1.3.1.20 | 0.0183 | - |
trans-benzene dihydrodiol | DD2 | Homo sapiens | |
| 1.3.1.20 | 0.0183 | - |
androsterone | DD4 | Homo sapiens | |
| 1.3.1.20 | 0.0333 | - |
trans-benzene dihydrodiol | DD4 | Homo sapiens | |
| 1.3.1.20 | 0.0483 | - |
trans-benzene dihydrodiol | DD1 | Homo sapiens | |
| 1.3.1.20 | 0.0867 | - |
(S)-indan-1-ol | 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | Homo sapiens | |
| 1.3.1.20 | 0.125 | - |
1-Acenaphthenol | DD4 | Homo sapiens | |
| 1.3.1.20 | 0.158 | - |
(S)-indan-1-ol | DD4 | Homo sapiens | |
| 1.3.1.20 | 0.163 | - |
(S)-indan-1-ol | DD2 | Homo sapiens | |
| 1.3.1.20 | 0.208 | - |
(S)-indan-1-ol | DD1 | Homo sapiens | |
| 1.3.1.20 | 0.417 | - |
1-Acenaphthenol | 3alpha-hydroxysteroid/dihydrodiol dehydrogenase | Homo sapiens | |
| 1.3.1.20 | 0.49 | - |
1-Acenaphthenol | DD1 | Homo sapiens | |
| 1.3.1.20 | 0.585 | - |
1-Acenaphthenol | DD2 | Homo sapiens | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.20 | NADP+ | - |
Homo sapiens | |
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