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Literature summary extracted from

  • Hoog, S.S.; Pawlowski, J.E.; Alzari, P.M.; Penning, T.M.; Lewis, M.
    Three-dimensional structure of rat liver 3.alpha-hydroxysteroid/dihydrodiol dehydrogenase: a member of the aldo-keto reductase superfamily (1994), Proc. Natl. Acad. Sci. USA, 91, 2517-2521.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.1.20 medicine enzyme can be used for screening of antiinflammatory potency of drugs Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.20 vapour diffusion at 22°C in 100 mM sodium citrate, polyethylene glycol 6000 25%, 120 mM ammonium sulfate, pH 5.8, x-ray analysis Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.20 C170A kinetically similar to wild-type Rattus norvegicus
1.3.1.20 C217A 4fold increase in Km for 5alpha-androstan-3,17-dione and 2fold increase in Km for NADH Rattus norvegicus
1.3.1.20 additional information sequence analysis Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.20 additional information inhibition by nonsteroidal antiinflammatory drugs Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.20 37029
-
 1 * 37029, cDNA sequence determination Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.20 additional information Rattus norvegicus reduces biologically important 3-oxo steroids of the androstane, pregnane, and cholane series ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.20 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ cofactor binding and catalytic triad, alpha/beta-barrel structure Rattus norvegicus
a
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ enzyme is multifunctional: 3alpha-hydroxysteroid dehydrogenase activity, EC 1.1.1.50, dihydrodiol dehydrogenase activity, EC 1.3.1.20, and 9-, 11-, and15-hydroxyprostaglandin dehydrogenase activity, belongs to aldo-keto reductase superfamily Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.20 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.20 3-keto steroid + NAD(P)H reduces biologically important 3-oxo steroids of the androstane, pregnane, and cholane series Rattus norvegicus 3alpha-hydroxy steroid + NAD(P)+
-
 ?
1.3.1.20 additional information reduces biologically important 3-oxo steroids of the androstane, pregnane, and cholane series Rattus norvegicus ?
-
 ?
1.3.1.20 additional information oxidation of trans-dihydrodiols of polycyclic hydrocarbons and in this way suppression of formation of anti-diol epoxid carcinogens Rattus norvegicus additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.20 monomer 1 * 37029, cDNA sequence determination Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.20 NADH
-
 Rattus norvegicus
1.3.1.20 NADP+
-
 Rattus norvegicus