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Literature summary extracted from

  • Kume, T.; Iwasa, H.; Shiraishi, H.; Yokoi, T.; Nagashima, K.; Otsuka, M.; Terada, T.; Takagi, T.; Hara, A.; Kamataki, T.
    Characterization of a novel variant (S145C/L311V) of 3alpha-hydroxysteroid/dihydrodiol dehydrogenase in human liver (1999), Pharmacogenetics, 9, 763-771.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.20 Sulfobromophthalein DD4 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.20 DD4 double mutant S145C/L311V overexpressed in Escherichia coli Homo sapiens
1.3.1.20 DD4, isolation of mRNA of native mutants isolated from a liver sample, synthesis of cDNA by RT-PCR, polymorphism of DD4 gene Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.20 L311V native mutant with C to G replacement in DNA at position 931 Homo sapiens
1.3.1.20 S145C native mutant with C to G replacement in DNA at position 434, no significant effect on activity Homo sapiens
1.3.1.20 S145C/L311V constructed double mutant with 3 to 5fold decreased activity for xenobiotic and steroidal substrates Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.20 Betamethasone DD4 wild-type and mutants Homo sapiens
1.3.1.20 dexamethasone DD4 wild-type and mutants Homo sapiens
1.3.1.20 Hexestrol DD4 wild-type and mutants Homo sapiens
1.3.1.20 indomethacin DD4 wild-type and mutants Homo sapiens
1.3.1.20 medroxyprogesterone DD4 wild-type and mutants Homo sapiens
1.3.1.20 additional information DD4 is inhibited by steroidal anti-inflammatory drugs Homo sapiens
1.3.1.20 Phenolphthalein DD4 wild-type and mutants Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.20 36000
-
 DD4 wild-type and mutant enzymes, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.20 additional information Homo sapiens involved in the metabolism of steroid hormones and polycyclic aromatic hydrocarbons, and also in the reduction of ketone-containing drugs ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.20 Homo sapiens
-
 DD4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.20 DD4 double mutant S145C/L311V recombinant from Escherichia coli Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ DD4 is identical with human chlordecone reductase and 3alpha-hydroxysteroid dehydrogenase type 1, EC 1.1.1.213, catalyzes the oxidoreduction of various 3alpha-hydroxysteroids including bile acids and belong to the aldo-keto reductase superfamily, in this concern termed AKR1C4 Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.20 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.20 0.73
-
 DD4 recombinant double mutant S145C/L311V, purified, substrate (S)-indan-1-ol Homo sapiens
1.3.1.20 1.5
-
 DD4 recombinant mutant S145C, purified, substrate (S)-indan-1-ol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.20 (R)-indan-1-ol + NADP+ DD4, recombinant double mutant S145C/L311V Homo sapiens ?
-
 ?
1.3.1.20 (R)-tetralol + NADP+ DD4, recombinant double mutant S145C/L311V Homo sapiens ?
-
 ?
1.3.1.20 (S)-indan-1-ol + NADP+ DD4, wild-type and recombinant double mutant S145C/L311V Homo sapiens ?
-
 ?
1.3.1.20 (S)-tetralol + NADP+ DD4, recombinant double mutant S145C/L311V Homo sapiens ?
-
 ?
1.3.1.20 3alpha-hydroxy steroid + NADP+
-
 Homo sapiens 3-keto steroid + NADPH
-
 ?
1.3.1.20 5beta-androstan-3alpha,17beta-diol + NADP+ DD4 wild-type and mutants Homo sapiens 5beta-androstan-3,17-dione + NADPH
-
 ?
1.3.1.20 androsterone + NADPH DD4 wild-type and mutants Homo sapiens ?
-
 ?
1.3.1.20 cholic acid + NADP+ DD4, wild-type and recombinant double mutant S145C/L311V Homo sapiens ?
-
 ?
1.3.1.20 additional information involved in the metabolism of steroid hormones and polycyclic aromatic hydrocarbons, and also in the reduction of ketone-containing drugs Homo sapiens ?
-
 ?
1.3.1.20 additional information DD4 also catalyzes oxidoreduction of 3alpha-hydroxysteroids and bile acids and is thus thought to be identical with 3alpha-hydroxysteroid dehydrogenase EC 1.1.1.213 Homo sapiens additional information
-
 ?
1.3.1.20 pyridine-3-aldehyde + NADPH DD4 wild-type and mutants Homo sapiens ?
-
 ?
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+
-
 Homo sapiens catechol + NAD(P)H
-
 ?
1.3.1.20 trans-naphthalene dihydrodiol + NAD(P)+
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.20 monomer 1 * 36000, DD4 wild-type and mutants, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.3.1.20 DD
-
 Homo sapiens
1.3.1.20 dihydrodiol dehydrogenase
-
 Homo sapiens
1.3.1.20 More DD4 shows only 83% sequence homology to the other human DD isoforms Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.20 25
-
 assay at, recombinant doule mutant Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.20 0.00667
-
 trans-benzene dihydrodiol double mutant 145C/L311V Homo sapiens
1.3.1.20 0.0117
-
 trans-benzene dihydrodiol
-
 Homo sapiens
1.3.1.20 0.045
-
 5beta-Androstan-3alpha,17beta-diol double mutant S145C/L311V Homo sapiens
1.3.1.20 0.0567
-
 (S)-indan-1-ol double mutant S145C/L311V Homo sapiens
1.3.1.20 0.0883
-
 cholic acid double mutant S145C/L311V Homo sapiens
1.3.1.20 0.108
-
 5beta-Androstan-3alpha,17beta-diol
-
 Homo sapiens
1.3.1.20 0.123
-
 (S)-indan-1-ol
-
 Homo sapiens
1.3.1.20 0.233
-
 cholic acid
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.20 NADP+
-
 Homo sapiens