EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.20 | expression of His-tagged protein in Escherichia coli | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.20 | C145S | same sensitivity to SH-reagents as wild-type | Bos taurus |
1.3.1.20 | C193S | no sensitivity to SH-reagents remaining | Bos taurus |
1.3.1.20 | D50N | low dehydrogenase activity | Bos taurus |
1.3.1.20 | H117Q | increased reductase activity | Bos taurus |
1.3.1.20 | K84N | low dehydrogenase activity, no remaining reductase activity | Bos taurus |
1.3.1.20 | Y55F | very low dehydrogenase activity | Bos taurus |
1.3.1.20 | Y55H | decreased dehydrogenase activity, significant increased in reductase activity | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.20 | 5,5'-dithiobis(2-nitrobenzoic acid) | i.e. DTNB, inhibits wild-type enzyme and mutant C145S | Bos taurus | |
1.3.1.20 | glutathione | wild-type and mutant C145S | Bos taurus | |
1.3.1.20 | N-ethylmaleimide | wild-type and mutant C145S | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.20 | additional information | Bos taurus | metabolic inactivation of exogeneous xenobiotics | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.20 | Bos taurus | - |
isoform DD3 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.20 | DD3, recombinant, His-tagged protein from Escherichia coli | Bos taurus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.3.1.20 | trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | structure analysis reveals that the enzyme belongs to the aldo-keto reductase superfamily, like 3alpha-hydroxysteroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase, 20alpha-hydroxysteroid dehydrogenase, aldose reductase, and aldehyde reductase | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.20 | liver | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.20 | additional information | - |
- |
Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.20 | 1-acenaphthenol + NAD(P)+ | - |
Bos taurus | ? | - |
? | |
1.3.1.20 | camphorquinone + NAD(P)H | - |
Bos taurus | (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+ | - |
? | |
1.3.1.20 | additional information | metabolic inactivation of exogeneous xenobiotics | Bos taurus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.20 | DD | - |
Bos taurus |
1.3.1.20 | dihydrodiol dehydrogenase | - |
Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.20 | 9.5 | - |
mutant H117Q of DD3 | Bos taurus |
1.3.1.20 | 9.7 | 10.5 | mutant K84N of DD3 | Bos taurus |
1.3.1.20 | 10 | - |
mutant D50N of DD3 | Bos taurus |
1.3.1.20 | 10.5 | - |
DD3, wild-type | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.20 | NADP+ | - |
Bos taurus | |
1.3.1.20 | NADPH | - |
Bos taurus |