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Literature summary extracted from

  • Terada, T.; Fujita, N.; Sugihara, Y.; Sato, R.; Takagi, T.; Maeda, M.
    Site-directed mutagenesis studies of bovine liver cytosolic dihydrodiol dehydrogenase: the role of Asp-50, Tyr-55, Lys-84, His-117, Cys-145 and Cys-193 in enzymatic activity (2001), Chem. Biol. Interact., 130-132, 833-845.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.20 expression of His-tagged protein in Escherichia coli Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.20 C145S same sensitivity to SH-reagents as wild-type Bos taurus
1.3.1.20 C193S no sensitivity to SH-reagents remaining Bos taurus
1.3.1.20 D50N low dehydrogenase activity Bos taurus
1.3.1.20 H117Q increased reductase activity Bos taurus
1.3.1.20 K84N low dehydrogenase activity, no remaining reductase activity Bos taurus
1.3.1.20 Y55F very low dehydrogenase activity Bos taurus
1.3.1.20 Y55H decreased dehydrogenase activity, significant increased in reductase activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.20 5,5'-dithiobis(2-nitrobenzoic acid) i.e. DTNB, inhibits wild-type enzyme and mutant C145S Bos taurus
1.3.1.20 glutathione wild-type and mutant C145S Bos taurus
1.3.1.20 N-ethylmaleimide wild-type and mutant C145S Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.20 additional information Bos taurus metabolic inactivation of exogeneous xenobiotics ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.20 Bos taurus
-
isoform DD3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.20 DD3, recombinant, His-tagged protein from Escherichia coli Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ structure analysis reveals that the enzyme belongs to the aldo-keto reductase superfamily, like 3alpha-hydroxysteroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase, 20alpha-hydroxysteroid dehydrogenase, aldose reductase, and aldehyde reductase Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.20 liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.20 additional information
-
-
Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.20 1-acenaphthenol + NAD(P)+
-
Bos taurus ?
-
?
1.3.1.20 camphorquinone + NAD(P)H
-
Bos taurus (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
?
1.3.1.20 additional information metabolic inactivation of exogeneous xenobiotics Bos taurus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.20 DD
-
Bos taurus
1.3.1.20 dihydrodiol dehydrogenase
-
Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.20 9.5
-
mutant H117Q of DD3 Bos taurus
1.3.1.20 9.7 10.5 mutant K84N of DD3 Bos taurus
1.3.1.20 10
-
mutant D50N of DD3 Bos taurus
1.3.1.20 10.5
-
DD3, wild-type Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.20 NADP+
-
Bos taurus
1.3.1.20 NADPH
-
Bos taurus