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Literature summary extracted from

  • Hara, A.; Harada, T.; Nakagawa, M.; Matsuura, K.; Nakayama, T.; Sawada, H.
    Isolation from pig lens of two proteins with dihydrodiol dehydrogenase and aldehyde reductase activities (1989), Biochem. J., 264, 403-407.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.20 diphosphate high concentration: F2 slight inhibition, F1 stimulation Sus scrofa
1.3.1.20 NaCl high concentration: F2 inhibition, F1 stimulation Sus scrofa
1.3.1.20 SO42- stimulates isoform F2 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.20 Barbital inhibits isoform F2 Sus scrofa
1.3.1.20 cyclopentane-1,1-diacetic acid NaCl inhibits only isoform F2 Sus scrofa
1.3.1.20 diphosphate high concentration: F2 slight inhibition, F1 stimulation Sus scrofa
1.3.1.20 Hexestrol i.e. 4,4'-(1,2-diethyl-1,2-ethanediyl)bisphenol, inhibits isoform F2 Sus scrofa
1.3.1.20 indomethacin inhibits both isoforms Sus scrofa
1.3.1.20 NaCl high concentration: F2 inhibition, F1 stimulation Sus scrofa
1.3.1.20 p-chloromercuribenzene sulfonate stronger effect on isoform F2 Sus scrofa
1.3.1.20 p-chloromercuribenzoate stronger effect on isoform F1 Sus scrofa
1.3.1.20 quercitrin inhibits isoform F2 and slightly isoform F1 Sus scrofa
1.3.1.20 SO42- inhibits only isoform F1 Sus scrofa
1.3.1.20 sorbinil inhibits isoform F1 Sus scrofa
1.3.1.20 Thiol-blocking reagents F1 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.20 35000
-
 1 * 35000, F2, SDS-PAGE Sus scrofa
1.3.1.20 39000
-
 2 * 39000, F1, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.20 naphthalene dihydrodiol + NAD(P)+ Sus scrofa
-
 ?
-
 ?
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+ Sus scrofa
-
 catechol + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.20 Sus scrofa
-
 at least 2 proteins: monomeric form F2 and dimeric form F1 with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2, F1 shows higher dihydrodiol activity, F2 shows higher aldehyde reductase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.20 at least 2 proteins: monomeric form F2 and dimeric form F1 with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2 Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ probably identical with aldehyde reductase EC 1.1.1.2 Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.20 adrenal gland low activity Sus scrofa
-
1.3.1.20 brain low activity Sus scrofa
-
1.3.1.20 lens
-
 Sus scrofa
-
1.3.1.20 lung low activity Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.20 DL-glyceraldehyde + NADPH probable identity with aldehyde reductase EC 1.1.1.2 Sus scrofa ?
-
 ?
1.3.1.20 additional information at least 2 proteins, monomeric form F2 and dimeric form F1, with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2, F1: specifically oxidizes trans-hydrodiols of naphthalene and benzene strictly with NADP+ as cofactor, reduction of vicinal diketones, aromatic aldehydes and glyceraldes with NADPH as cofactor Sus scrofa additional information
-
 ?
1.3.1.20 additional information not: hydroxy steroids Sus scrofa additional information
-
 ?
1.3.1.20 additional information F2: high activity towards naphthalene dihydrodiol and some alcohols, low activity towards trans- and cis-benzene dihydrodiols and some alcohols, reduction of aldoses, vicinal diketones, aromatic aldehydes and glyceraldehydes with NADPH or NADH as cofactors Sus scrofa additional information
-
 ?
1.3.1.20 naphthalene dihydrodiol + NAD(P)+
-
 Sus scrofa ?
-
 ?
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+
-
 Sus scrofa catechol + NAD(P)H
-
 ?
1.3.1.20 trans-naphthalene dihydrodiol + NAD(P)+
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.20 dimer 2 * 39000, F1, SDS-PAGE Sus scrofa
1.3.1.20 monomer 1 * 35000, F2, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.20 25
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.20 5.8
-
 F2: reduction of vicinal diketones, aromatic aldehydes and glyceraldehydes + NAD(P)H Sus scrofa
1.3.1.20 6 8 F1: reduction of vicinal diketones, aromatic aldehydes and glyceraldehydes + NADPH Sus scrofa
1.3.1.20 7.6
-
 F2: oxidation of naphthalene dihydrodiol + NAD(P)+ Sus scrofa
1.3.1.20 10.2
-
 F1: oxidation of trans-dihydrodiols of naphthalene and benzene + NADP+ Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.20 NAD+
-
 Sus scrofa
1.3.1.20 NADH activity only with isoform F2 Sus scrofa
1.3.1.20 NADP+
-
 Sus scrofa
1.3.1.20 NADPH
-
 Sus scrofa