Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dobritzsch, D.; Ricagno, S.; Schneider, G.; Schnackerz, K.D.; Lindqvist, Y.
    Crystal structure of the productive ternary complex of dihydropyrimidine dehydrogenase with NADPH and 5-iodouracil. Implications for mechanism of inhibition and electron transfer (2002), J. Biol. Chem., 277, 13155-13166.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.2 expression in Escherichia coli Sus scrofa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.2 cocrystallization with 5-iodouracil 1 mM and NADPH 5 mM, ternary complex in 100 mM HEPES, pH 7.5, 22% polyethylene glycol 6000, uracil-4-acetic acid 1 mM, structure determination at different pH-values Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.2 5-Iodouracil inhibition mechanism Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.2 cytoplasm
-
 Sus scrofa 5737
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.2 recombinant protein Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.2 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 thymine + NADPH
-
 Sus scrofa dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH
-
 Sus scrofa 5,6-dihydrouracil + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 NADPH
-
 Sus scrofa