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Literature summary extracted from

  • Porter, D.J.T.; Chestnut, W.G.; Taylor, L.C.E.; Merrill, B.M.; Spector, T.
    Inactivation of dihydropyrimidine dehydrogenase by 5-iodouracil (1991), J. Biol. Chem., 266, 19988-19994.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.2 dithiothreitol stabilizes against inactivation via 5-alkylyluracils Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.2 5-Iodouracil dithiothreitol and thymine protect against inactivation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.2 thymine + NADPH Bos taurus rate-limiting enzyme in catabolic detoxification of exogenous fluoropyrimidines dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH Bos taurus
-
 5,6-dihydrouracil + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.2 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.2 0.38
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 5-fluorouracil + NADPH + H+
-
 Bos taurus 5-fluoro-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 5-iodouracil + NADPH
-
 Bos taurus 5-iodo-5,6-dihydrouracil + NADP+ alkylating and therefore in vivo inactivating product ?
1.3.1.2 thymine + NADPH
-
 Bos taurus dihydrothymine + NADP+
-
 ?
1.3.1.2 thymine + NADPH rate-limiting enzyme in catabolic detoxification of exogenous fluoropyrimidines Bos taurus dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH
-
 Bos taurus 5,6-dihydrouracil + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.2 37
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 NADP+
-
 Bos taurus
1.3.1.2 NADPH
-
 Bos taurus