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Literature summary extracted from
- Pyrimidine reducing enzymes of rat liver (1976), Can. J. Biochem., 54, 178-184.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.1.1 | cytosol | - |
Rattus norvegicus | 5829 | - |
| 1.3.1.1 | mitochondrion | low content | Rattus norvegicus | 5739 | - |
| 1.3.1.2 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
| 1.3.1.2 | endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.2 | 5,6-dihydrouracil + NADP+ | Rattus norvegicus | - |
uracil + NADPH | - |
ir |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.1 | Rattus norvegicus | - |
- |
- |
| 1.3.1.2 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.1 | - |
Rattus norvegicus |
| 1.3.1.2 | partial | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.3.1.1 | liver | - |
Rattus norvegicus | - |
| 1.3.1.2 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.1 | 0.535 | - |
NADH, uracil reduction | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.1 | thymine + NAD(P)H + H+ | - |
Rattus norvegicus | dihydrothymine + NAD(P)+ | - |
r | |
| 1.3.1.1 | uracil + NAD(P)H + H+ | preferred substrate | Rattus norvegicus | 5,6-dihydrouracil + NAD(P)+ | - |
? | |
| 1.3.1.2 | 5,6-dihydrouracil + NADP+ | - |
Rattus norvegicus | uracil + NADPH | - |
ir | |
| 1.3.1.2 | 5,6-dihydrouracil + NADP+ | reversibility of the reaction only in vitro | Rattus norvegicus | uracil + NADPH | - |
r | |
| 1.3.1.2 | dihydrothymine + NADP+ | - |
Rattus norvegicus | thymine + NADPH | - |
r | |
| 1.3.1.2 | thymine + NADPH | - |
Rattus norvegicus | dihydrothymine + NADP+ | - |
r | |
| 1.3.1.2 | uracil + NADPH | reversibility of the reaction only in vitro | Rattus norvegicus | 5,6-dihydrouracil + NADP+ | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.1 | 7.4 | - |
- |
Rattus norvegicus |
| 1.3.1.2 | 7.4 | - |
- |
Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.1 | NAD+ | preferred | Rattus norvegicus | |
| 1.3.1.1 | NADH | preferred | Rattus norvegicus | |
| 1.3.1.1 | NADP+ | less activity than with NAD+ | Rattus norvegicus | |
| 1.3.1.1 | NADPH | less activity than with NADH | Rattus norvegicus | |
| 1.3.1.2 | NAD+ | less activity than with NADP+, high concentration necessary | Rattus norvegicus | |
| 1.3.1.2 | NADH | less activity than with NADPH, high concentration necessary | Rattus norvegicus | |
| 1.3.1.2 | NADP+ | - |
Rattus norvegicus | |
| 1.3.1.2 | NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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