Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, Y.; Hawes, J.; Popov, K.M.; Jaskiewicz, J.; Shimomura, Y.; Crabb, D.W.; Harris, R.A.
    Site-directed mutagenesis of phosphorylation sites of the branched chain alpha-ketoacid dehydrogenase complex (1994), J. Biol. Chem., 269, 18583-18587.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.4.4 thiamine diphosphate
-
Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.4.4
-
Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.2.4.4 S293A mutation in the alpha-subunit, nearly 12fold increase in Km-value, no inactivation after incubation with ATP Rattus norvegicus
1.2.4.4 S293E mutation in the alpha-subunit, completely inactive enzyme Rattus norvegicus
1.2.4.4 S293E/S303E mutation in the alpha-subunit, no activity Rattus norvegicus
1.2.4.4 S303A mutation in the alpha-subunit, no effect upon enzyme activity Rattus norvegicus
1.2.4.4 S303E mutation in the alpha-subunit, no effect upon enzyme activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.4.4 ATP time-dependent inactivation of wild-type enzyme and mutant proteins S303A, S303E, with mutation in the alpha-subunit Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.4 160000
-
wild-type enzyme and mutant enzymes S293E, S293A, S303A and S293E/S303E, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.4
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.4 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Rattus norvegicus [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.4 CoA
-
Rattus norvegicus
1.2.4.4 NAD+
-
Rattus norvegicus