EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.4 | L-Val | activates, when ommited from the assay mixture the rate of NAD+ reduction decreases to 41% of the rate of the complete system | Pseudomonas putida | |
1.2.4.4 | thiamine diphosphate | required | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.4 | 0.033 | - |
coenzyme A | - |
Pseudomonas putida | |
1.2.4.4 | 0.12 | - |
NAD+ | reaction with 3-methyl-2-oxobutanoate | Pseudomonas putida | |
1.2.4.4 | 0.46 | - |
3-methyl-2-oxobutanoate | - |
Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.4 | Mg2+ | required | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.4.4 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.4.4 | multienzyme complex | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.4.4 | 10.1 | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.4.4 | 0-5°C, rapid loss of 20-30% of the activity, then stable for 3-4 months | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.4.4 | 2-oxoglutarate + NAD+ + CoA | no activity | Pseudomonas putida | 3-carboxypropionyl-CoA + NADH + CO2 | - |
? | |
1.2.4.4 | 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Pseudomonas putida | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 | - |
? | |
1.2.4.4 | 3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Pseudomonas putida | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2 | - |
? | |
1.2.4.4 | 4-methyl-2-oxopentanoate + NAD+ + CoA | - |
Pseudomonas putida | 3-methylbutanoyl-CoA + CO2 + NADH | - |
? | |
1.2.4.4 | pyruvate + NAD+ + CoA | no activity | Pseudomonas putida | acetyl-CoA + CO2 + NADH | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.4.4 | 7 | - |
- |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.4 | CoA | - |
Pseudomonas putida | |
1.2.4.4 | NAD+ | - |
Pseudomonas putida |