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Literature summary extracted from
- Purification, resolution, and reconstitution of branched-chain 2-keto acid dehydrogenase complex from bovine kidney (1988), Methods Enzymol., 166, 303-308.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.4.4 | thiamine diphosphate | - |
Bos taurus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.4.4 | 35000 | - |
alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE | Bos taurus |
| 1.2.4.4 | 46000 | - |
alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.4.4 | additional information | Bos taurus | the activity of the multienzyme complex is regulated by reversible phosphorylation of the alpha-subunit of the E1 component, EC 1.2.4.4. | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.4.4 | Bos taurus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.2.4.4 | kidney | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.4.4 | additional information | - |
- |
Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.2.4.4 | -20°C, after concentration and dialysis into 50 mM Tris-HCl buffer, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM benzamidine, 1 mM PMSF, 1 mM DTT, pH 7.3, multienzyme complex is stable for at least 2 months | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.4.4 | additional information | the activity of the multienzyme complex is regulated by reversible phosphorylation of the alpha-subunit of the E1 component, EC 1.2.4.4. | Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.4.4 | tetramer | alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.4.4 | CoA | - |
Bos taurus | |
| 1.2.4.4 | NAD+ | - |
Bos taurus | |
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