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Literature summary extracted from

  • Bessam, H.; Mareck, A.M.; Foucher, B.
    Neurospora crassa alpha-ketoglutarate dehydrogenase complex: description, resolution of components and catalytic properties (1989), Biochim. Biophys. Acta, 990, 66-72.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.105 successive freeze-thawing cycles of the 2-oxoglutarate dehydrogenase complex leads to complete inactivation Neurospora crassa
1.2.1.105 vigorous sonication of the 2-oxoglutarate dehydrogenase complex lead to complete inactivation Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.105 0.07
-
 2-oxoglutarate in presence of 0.0045 mM Ca2+ Neurospora crassa
1.2.1.105 0.47
-
 2-oxoglutarate in presence of 0.0225 mM Ca2+ Neurospora crassa
1.2.1.105 0.58
-
 2-oxoglutarate in presence of 0.0023 mM Ca2+ Neurospora crassa
1.2.1.105 0.75
-
 2-oxoglutarate in presence of 0.07 mM Ca2+ Neurospora crassa
1.2.1.105 0.8
-
 2-oxoglutarate without Ca2+ Neurospora crassa
1.2.1.105 0.84
-
 2-oxoglutarate in presence of 0.0012 mM Ca2+ Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.105 Ca2+ diminishes the Km value of the 2-oxoglutarate dehydrogenase complex for 2-oxoglutarate in the physiological range, maximal activation, 35%, with 0.004 mM Ca2+ Neurospora crassa
1.2.1.105 Mg2+ maximal activation, 75%, with 5 mM Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.105 52900
-
 x * 52900, 2-oxoglutarate dehydrogenase subunit E1, SDS-PAGE Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.105 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.105 2-oxoglutarate dehydrogenase complex Neurospora crassa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.105 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 2-oxoglutarate dehydrogenase complex consists of 3 enzymes: E1 (alpha-ketoglutarate dehydrogenase, EC 1.2.4.2), E2 (dihydrolipoyl transsuccinylase, EC 2.3.1.61), E3 (dihydrolipoyl dehydrogenase, EC 1.8.1.4) Neurospora crassa
a
1.2.1.105 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Neurospora crassa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.105 additional information
-
 specific activity of the 2-oxoglutarate dehydrogenase complex Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.105 2-oxoglutarate + lipoamide
-
 Neurospora crassa S-succinyldihydrolipoamide + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.105 ? x * 52900, 2-oxoglutarate dehydrogenase subunit E1, SDS-PAGE Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.105 35
-
 2-oxoglutarate dehydrogenase complex Neurospora crassa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.105 20 45 20°C: about 35% of maximal activity, 45°C: about 20% of maximal activity, 2-oxoglutarate dehydrogenase complex Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.105 4
-
 60 min, stable, 2-oxoglutarate dehydrogenase complex Neurospora crassa
1.2.1.105 21
-
 60 min, about 65% loss of activity, 2-oxoglutarate dehydrogenase complex Neurospora crassa
1.2.1.105 30
-
 60min, about 80% loss of activity, 2-oxoglutarate dehydrogenase complex Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.105 7.5
-
 2-oxoglutarate dehydrogenase complex Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.105 6.5 8.5 pH 6.5: about 25% of maximal activity, pH 8.5: about 30% of maximal activity, 2-oxoglutarate dehydrogenase complex Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.105 thiamine diphosphate required , 2-oxoglutarate dehydrogenase complex Neurospora crassa