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Literature summary extracted from

  • Karam, G.A.; Bishop, S.H.
    alpha-Ketoglutarate dehydrogenase from cauliflower mitochondria: preparation and reactivity with substrates (1989), Phytochemistry, 28, 3291-3293.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.4.2 0.116
-
 2-oxoglutarate pH 7.0 Brassica oleracea
1.2.4.2 0.564
-
 2-oxoglutarate pH 7.4 Brassica oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.4.2 mitochondrion
-
 Brassica oleracea 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.2 Brassica oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.2 partial, 2-oxoglutarate dehydrogenase complex Brassica oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 2-oxoglutarate dehydrogenase complex consists of 3 enzymes: E1 (alpha-ketoglutarate dehydrogenase, EC 1.2.4.2), E2 (dihydrolipoyl transsuccinylase, EC 2.3.1.61), E3 (dihydrolipoyl dehydrogenase, EC 1.8.1.4) Brassica oleracea
a
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Brassica oleracea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.4.2 floret
-
 Brassica oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.2 2-oxoglutarate + lipoamide
-
 Brassica oleracea S-succinyldihydrolipoamide + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.4.2 7.4
-
-
 Brassica oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.2 thiamine diphosphate required , 2-oxoglutarate dehydrogenase complex Brassica oleracea