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Literature summary extracted from

  • Henderson, C.E.; Perham, R.N.
    Purificaton of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus and resolution of its four component polypeptides (1980), Biochem. J., 189, 161-172.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.12 57000
-
 SDS-PAGE Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Geobacillus stearothermophilus
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Geobacillus stearothermophilus
-
-
-
2.3.1.12 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104 alpha and beta subunits purified from enzyme complex after gel filtration in the presence of 2 M KI followed by chromatography on hydroxyapatite in the presence of 8 M urea Geobacillus stearothermophilus
1.2.1.104 pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 Geobacillus stearothermophilus
2.3.1.12
-
 Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
1.2.1.104 -15°C, 50 mM potassium phosphate buffer, pH 7.0, 2 mM EDTA, 0.15 mM phenylmethanesulfonylfluoride, 0.2 g/l NaN3, several months Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Geobacillus stearothermophilus S-acetyldihydrolipoamide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.12 polymer stoichiometry of pyruvate dehydrogenase complexes Geobacillus stearothermophilus