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Literature summary extracted from

  • Pettit, F.H.; Reed, L.J.
    Pyruvate dehydrogenase complex from bovine kidney and heart (1982), Methods Enzymol., 89, 376-386.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.4.1 very sensitive to freezing and thawing Bos taurus
1.2.4.1 very sensitive to proteolysis, especially during purification Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.4.1 0.017
-
 pyruvate similar values Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.4.1 mitochondrion
-
 Bos taurus 5739
-
2.3.1.12 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.1 additional information
-
 MW of native complex isolated from kidney and heart: 7000000 Da and 8500000 Da respectively Bos taurus
1.2.4.1 36000
-
 alpha2,beta2, 2 * 41000 + 2 * 36000 Bos taurus
1.2.4.1 41000
-
 alpha2,beta2, 2 * 41000 + 2 * 36000 Bos taurus
1.2.4.1 154000
-
-
 Bos taurus
2.3.1.12 74000
-
 SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.4.1 pyruvate + lipoamide Bos taurus specific for S-acetyldihydrolipoamide + CO2
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA Bos taurus
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.1 Bos taurus
-
-
-
2.3.1.12 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.1 pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 Bos taurus
2.3.1.12 from pyruvate dehydrogenase complex Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.4.1 heart
-
 Bos taurus
-
1.2.4.1 kidney
-
 Bos taurus
-
2.3.1.12 heart
-
 Bos taurus
-
2.3.1.12 kidney
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.2.4.1 -50°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 20 mM thiamine diphosphate, 0.1 mM EDTA, 0.4 mM DTT, 0.1 mM NAD+, several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.1 pyruvate + lipoamide specific for Bos taurus S-acetyldihydrolipoamide + CO2
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Bos taurus S-acetyldihydrolipoamide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.4.1 tetramer alpha2,beta2, 2 * 41000 + 2 * 36000 Bos taurus